Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Fluorescence Binding Assays from Article 10.1021/acschembio.6b00720: "Molecular Mechanism for Isoform-Selective Inhibition of Acyl Protein Thioesterases 1 and 2 (APT1 and APT2)."
Assay data:3 Active, 2 Activity ≤ 1 µM, 4 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Steady-State Kinetic Assays from Article 10.1021/acschembio.6b00720: "Molecular Mechanism for Isoform-Selective Inhibition of Acyl Protein Thioesterases 1 and 2 (APT1 and APT2)."
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Enzymatic Protein Activity Assay from Article 10.1002/cbic.201200571: "Boron-based inhibitors of acyl protein thioesterases 1 and 2."
Assay data:14 Active, 5 Activity ≤ 1 µM, 18 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of APT-1/2 in mouse brain cytosolic fraction at 1 uM preincubated for 1 hr followed by TAMRA-Lys(biotin)-NH-propyne addition and measured after 1 hr click chemistry labelling based Western blot analysis
Assay data:2 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of APT-1/2 in mouse brain membrane fraction at 1 uM preincubated for 1 hr followed by TAMRA-Lys(biotin)-NH-propyne addition and measured after 1 hr click chemistry labelling based Western blot analysis
Inhibition of APT-1/2 in mouse brain cytosolic fraction at 0.1 to 1 uM preincubated for 1 hr followed by TAMRA-alkyne addition and measured after 1 hr by SDS-PAGE analysis based click chemistry labelling method
Inhibition of APT-1/2 in mouse brain membrane fraction at 0.1 to 1 uM preincubated for 1 hr followed by TAMRA-alkyne addition and measured after 1 hr by SDS-PAGE analysis based click chemistry labelling method
Inhibition of FP-Rh binding to APT-1/2 in human A-431 cells at 1 mM preincubated for 2 hrs followed by compound washout and subsequent FP-Rh addition and measured after 1 hr by SDS-PAGE analysis
Inhibition of FP-Rh binding to APT-1/2 in mouse brain cytosolic fraction at 10 uM preincubated for 1 hr followed by FP-Rh addition and measured after 1 hr in presence of APT1/2 inhibitor JCP-174-alk by SDS-PAGE analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of FP-Rh binding to APT-1/2 in mouse brain membrane fraction preincubated for 1 hr followed by FP-Rh addition and measured after 1 hr by SDS-PAGE analysis
Assay data:5 Active, 5 Tested
Inhibition of FP-Rh binding to APT-1/2 in mouse brain cytosolic fraction preincubated for 1 hr followed by FP-Rh addition and measured after 1 hr by SDS-PAGE analysis
Assay data:5 Active, 3 Activity ≤ 1 µM, 5 Tested
Binding affinity to LYPLA2 in rat cerebellar membranes at 10 uM measured after 1 hr by fluorescence based ABPP analysis
Assay data:1 Active, 1 Tested
Binding affinity to LYPLA1 in rat cerebellar membranes at 10 uM measured after 1 hr by fluorescence based ABPP analysis
Binding affinity to HEK293T cells-derived His-tagged APT1 expressed in Escherichia coli BL21(DE3) assessed as stoichiometric complex formation at 10 uM after 5 mins by electrospray ionization native mass spectrometry
Assay data:2 Tested
Binding affinity to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) assessed as stoichiometric complex formation at 10 uM after 5 mins by electrospray ionization native mass spectrometry
Inhibition of fluorescein-labeled ML349 binding to HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) after 30 mins by fluorescence polarization assay
Assay data:1 Active, 2 Tested
Binding affinity to APT2 in HEK293T cells at 50 uM incubated for 1 hr presence of generic lipase inhibitor HDFP by mass spectrometry analysis
Binding affinity to APT2 in HEK293T cells at 50 uM incubated for 1 hr by mass spectrometry analysis relative to azide-PEG3-biotin
Competitive inhibition of HEK293T cells-derived His-tagged APT2 expressed in Escherichia coli BL21(DE3) preincubated for 30 mins followed by substrate addition by resorufin dye based acetate hydrolysis assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 4 Tested
Binding affinity to APT2 in HEK293T cells at 50 uM incubated for 1 hr by mass spectrometry analysis relative to o-ML349-biotin
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on