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Inhibition of his tagged Escherichia coli K12 MurA C115D mutant using UDP-N-acetylglucosamine as substrate in presence of DTT and PEP by microplate reader analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Uncompetitive inhibition of wild type his tagged Escherichia coli K12 MurA at 2.23 to 3.34 uM using varying concentration of UDP-N-acetylglucosamine as substrate measured after 5 to 30 mins by malachite green assay
Inhibition of his tagged Escherichia coli K12 MurA C115D mutant at 2 uM using UDP-N-acetylglucosamine as substrate in presence of DTT, PEP and fosfomycin by microplate reader analysis relative to control
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of wild type his tagged Escherichia coli K12 MurA C115D mutant at 2 uM using UDP-N-acetylglucosamine as substrate in presence of DTT and PEP by microplate reader analysis relative to control
Assay data:2 Tested
Inhibition of wild type his tagged Escherichia coli K12 MurA at 2 uM using UDP-N-acetylglucosamine as substrate preincubated with substrate for 30 mins prior to compound addition for 15 mins followed by DTT and PEP addition and measured after 30 mins in presence of fosfomycin by malachite green based microplate reader analysis relative to control
Inhibition of wild type his tagged Escherichia coli K12 MurA at 2 uM using UDP-N-acetylglucosamine as substrate preincubated with substrate for 30 mins prior to compound addition for 15 mins followed by DTT and PEP addition and measured after 30 mins by malachite green based microplate reader analysis relative to control
Inhibition of wild type his tagged Escherichia coli K12 MurA at 0.2 uM using UDP-N-acetylglucosamine as substrate preincubated with substrate for 15 mins prior to compound addition for 15 mins followed by DTT and PEP addition by dilution assay
Assay data:1 Active, 2 Tested
Inhibition of wild type his tagged Escherichia coli K12 MurA using UDP-N-acetylglucosamine as substrate preincubated with substrate for 30 mins prior to compound addition for 15 mins followed by DTT and PEP addition and measured after 30 mins by malachite green based microplate reader analysis
Assay data:12 Active, 41 Tested
Inhibition of wild type his tagged Escherichia coli K12 MurA at 20 uM using UDP-N-acetylglucosamine as substrate preincubated with substrate for 30 mins prior to compound addition for 15 mins followed by DTT and PEP addition and measured after 30 mins by malachite green based microplate reader analysis relative to control
Assay data:47 Tested
Inhibition of recombinant Escherichia coli MurA assessed using UDP-N-acetylglucosamine as substrate preincubated for 30 mins in presence of 1 mM DTT followed by substrate addition and measured by malachite green based colorimetric assay
Assay data:14 Tested
Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 500 uM using UDP-N-acetylglucosamine as substrate preincubated for 30 mins in presence of 1 mM DTT followed by substrate addition and measured by malachite green based colorimetric assay relative to control
Assay data:49 Tested
Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 500 uM using UDP-N-acetylglucosamine as substrate and measured by malachite green based colorimetric assay relative to control
Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 500 uM using UDP-N-acetylglucosamine as substrate preincubated for 30 mins followed by substrate addition and measured by malachite green based colorimetric assay relative to control
Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 0.5 mM using UDP-N-acetylglucosamine as substrate incubated for 15 mins and measured by malachite green based colorimetric assay relative to control
Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 0.1 mM using UDP-N-acetylglucosamine as substrate incubated for 15 mins and measured by malachite green based colorimetric assay relative to control
Inhibition of recombinant Escherichia coli MurA assessed as residual activity at 1 mM using UDP-N-acetylglucosamine as substrate incubated for 15 mins and measured by malachite green based colorimetric assay relative to control
Inhibition of recombinant Escherichia coli MurA using UDP-N-acetylglucosamine as substrate incubated for 15 mins and measured by malachite green based colorimetric assay
Assay data:5 Tested
Inhibition of GST-tagged Escherichia coli MurA expressed in Escherichia coli BL21(DE3) cells preincubated with PEP and enzyme for 10 mins followed by UDP-GlcNAc addition measured after 3 hrs by HPLC analysis
Inhibition of Escherichia coli K-12 MBP-fused C-terminal His-tagged MurA (6508 to 7768 residues) using UNAG as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins in presence of PEP by malachite green dye based spectrophotometric method
Experimentally measured binding affinity data (Ki) for protein-ligand complexes derived from PDB
Assay data:3598 Active, 421 Activity ≤ 1 nM, 2233 Activity ≤ 1 µM, 3598 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
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