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Inhibition of mouse RhoA assessed as decrease in B-max for GTP-binding on Rac1 by mant-GTP binding kinetics real-time assay
Assay data:1 Tested
SummaryRelated BioAssays by Target
Binding affinity to mouse RhoA by SPR assay
Inhibition of human p190 expressed in mouse NIH/3T3 cells assessed as reduction in human full length RhoA (1 to 193 residues) interaction with p190 at 50 to 100 uM incubated for 1 hr by Western blot analysis based His-RhoA pull-down assay
Inhibition of human mDia1 expressed in mouse NIH/3T3 cells assessed as reduction in human full length RhoA (1 to 193 residues) interaction with mDia1 at 50 to 100 uM incubated for 1 hr by Western blot analysis based His-RhoA pull-down assay
Inhibition of human ROCK2 expressed in mouse NIH/3T3 cells assessed as reduction in human full length RhoA (1 to 193 residues) interaction with ROCK2 at 50 to 200 uM incubated for 1 hr by Western blot analysis based His-RhoA pull-down assay
Inhibition of FLAG-tagged human LBC RhoGEF DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in human full length RhoA (1 to 193 residues) interaction with LBC RhoGEF at 10 to 50 uM incubated for 1 hr by Western blot analysis based His-RhoA pull-down assay
Inhibition of GST-tagged human DBL RhoGEF DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in human full length RhoA (1 to 193 residues) interaction with DBL RhoGEF at 10 to 50 uM incubated for 1 hr by Western blot analysis based His-RhoA pull-down assay
Inhibition of FLAG-tagged human PDZ RhoGEF DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in human full length RhoA (1 to 193 residues) interaction with PDZ RhoGEF at 30 to 50 uM incubated for 1 hr by Western blot analysis based GST-RhoA pull-down assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of GFP-tagged human p115 RhoGEF DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in human full length RhoA (1 to 193 residues) interaction with p115 RhoGEF at 30 to 50 uM incubated for 1 hr by Western blot analysis based GST-RhoA pull-down assay
Inhibition of constitutively active RhoA Q63L mutant (unknown origin) expressed in mouse NIH/3T3 cells assessed as reduction in 10% calf serum-induced actin stress fiber formation at 50 uM by rhodamine-phalloidin staining based assay
Inhibition of constitutively active RhoA Q63L mutant (unknown origin) expressed in mouse NIH/3T3 cells assessed as reduction in 10% calf serum-induced focal adhesion complex formation at 50 uM by anti-vinculin antibody staining based assay
Inhibition of constitutively active RhoA Q63L mutant (unknown origin) expressed in mouse NIH/3T3 cells assessed as reduction in 10% calf serum-induced phospho-MLC formation at 50 uM incubated for 24 hrs by densitometry
Binding affinity to human LARG (765 to 1138 residues) DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in LARG binding to human full length RhoA (1 to 193 residues) by measuring relative LARG binding level at 5 uM pull-down assay (Rvb = 1 No unit)
Assay data:2 Tested
Binding affinity to human LARG (765 to 1138 residues) DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in LARG binding to human full length RhoA (1 to 193 residues) by measuring relative LARG binding level at 10 uM pull-down assay (Rvb = 1 No unit)
Binding affinity to human LARG (765 to 1138 residues) DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in LARG binding to human full length RhoA (1 to 193 residues) by measuring relative LARG binding level at 30 uM pull-down assay (Rvb = 1 No unit)
Binding affinity to human LARG (765 to 1138 residues) DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in LARG binding to human full length RhoA (1 to 193 residues) by measuring relative LARG binding level at 50 uM pull-down assay (Rvb = 1 No unit)
Binding affinity to human LARG (765 to 1138 residues) DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in LARG binding to human full length RhoA (1 to 193 residues) by measuring relative LARG binding level at 2.5 uM in presence of 2.5 uM Rhosin/G04 pull-down assay (Rvb = 1 No unit)
Binding affinity to human LARG (765 to 1138 residues) DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in LARG binding to human full length RhoA (1 to 193 residues) by measuring relative LARG binding level at 5 uM in presence of 5 uM Rhosin/G04 pull-down assay (Rvb = 1 No unit)
Binding affinity to human LARG (765 to 1138 residues) DH-PH module expressed in mouse NIH/3T3 cells assessed as reduction in LARG binding to human full length RhoA (1 to 193 residues) by measuring relative LARG binding level at 15 uM in presence of 15 uM Rhosin/G04 pull-down assay (Rvb = 1 No unit)
Inhibition of His6-tagged human LARG PH-DH domain expressed in mouse NIH/3T3 cells assessed as reduction in human full length GST-tagged RhoA (1 to 193 residues) interaction with LARG RhoGEF at 100 uM incubated for 1 hr by Western blot analysis relative to Y16
Assay data:5 Tested
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