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Irreversible inhibition of recombinant Streptococcus pyogenes sortase A deltaN81 expressed in Escherichia coli BL21(DE3) cells using Abz-LPATG-Dnp as substrate assessed as inactivation constant preincubated for 60 mins followed by substrate addition and measured every 1 min for 15 mins by fluorescence intensity based assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Irreversible inhibition of recombinant Streptococcus pyogenes sortase A deltaN81 expressed in Escherichia coli BL21(DE3) cells using Abz-LPATG-Dnp as substrate preincubated for 60 mins followed by substrate addition and measured every 1 min for 15 mins by FRET-based assay
Irreversible inhibition of recombinant Staphylococcus aureus sortase A deltaN24 expressed in Escherichia coli BL21(DE3) cells using Abz-LPATG-Dnp as substrate preincubated for 60 mins followed by substrate addition and measured every 1 min for 15 mins by FRET-based assay
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Irreversible inhibition of recombinant Staphylococcus aureus sortase A deltaN24 expressed in Escherichia coli BL21(DE3) cells using Abz-LPATG-Dnp as substrate preincubated for 40 mins followed by substrate addition and measured every 1 min for 15 mins by FRET-based assay
Irreversible inhibition of recombinant Staphylococcus aureus sortase A deltaN24 expressed in Escherichia coli BL21(DE3) cells using Abz-LPATG-Dnp as substrate preincubated for 20 mins followed by substrate addition and measured every 1 min for 15 mins by FRET-based assay
Inhibition of recombinant Staphylococcus aureus sortase A deltaN24 transpeptidation expressed in Escherichia coli BL21 (DE3) cells using Abz-LPATG-Dnp as substrate preincubated for 20 mins followed by substrate addition and measured every 1 min for 15 mins by FRET assay
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