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Inhibition of recombinant full-length wild type Symbiobacterium thermophilum S1PL using RBM13 as fluorogenic substrate incubated for 1 hr
Assay data:10 Tested
SummaryPubMed Citation
Inhibition of recombinant full-length wild type Symbiobacterium thermophilum S1PL at 250 uM using RBM13 as fluorogenic substrate incubated for 1 hr relative to control
Assay data:1 Tested
Activity at recombinant Symbiobacterium thermophilum S1PL expressed in Escherichia coli M-15 assessed as increase in substrate metabolism by fluorogenic assay relative to control
Inhibition of recombinant Symbiobacterium thermophilum S1PL expressed in Escherichia coli M-15 assessed as trans-2-hexacedenal formation at 50 uM using S1P as substrate after 1 hr by UPLC-MS analysis relative to control
Inhibition of recombinant Symbiobacterium thermophilum S1PL expressed in Escherichia coli M-15 at 125 uM using RBM13 as substrate measured after 1 hr by fluorogenic assay
Inhibition of recombinant Symbiobacterium thermophilum S1PL expressed in Escherichia coli M-15 at 250 uM using RBM13 as substrate measured after 1 hr by fluorogenic assay
Assay data:4 Active, 5 Tested
SummaryCompounds, ActivePubMed Citation
Ratio of Vmax to Km for recombinant Symbiobacterium thermophilum S1PL expressed in Escherichia coli M-15
Activity at recombinant Symbiobacterium thermophilum S1PL expressed in Escherichia coli M-15 after 60 mins by Michaelis-Menten plot analysis
Activity at recombinant Symbiobacterium thermophilum S1PL expressed in Escherichia coli M-15 assessed as umbelliferone formation by fluorogenic assay
Activity at recombinant Symbiobacterium thermophilum S1PL expressed in Escherichia coli M-15 assessed as substrate metabolism by fluorogenic assay
Activity at recombinant Symbiobacterium thermophilum S1PL expressed in Escherichia coli M-15 assessed as umbelliferone formation at 62 uM by fluorogenic assay
Assay data:1 Active, 1 Tested
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