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Inhibition of SrtA delta N24 mutant in Staphylococcus aureus subsp. aureus str. Newman reduction in abundance of anchored SpA at 50 to 200 uM by Western blot analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of SrtA delta N24 mutant in Staphylococcus aureus subsp. aureus str. Newman reduction in abundance of anchored SpA by Western blot analysis
Inhibition of SrtA delta N24 mutant in Staphylococcus aureus subsp. aureus str. Newman reduction in abundance of anchored SpA at 100 uM by FITC-labeled human IgG fluorescence based assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of SrtA delta N24 mutant in Staphylococcus aureus subsp. aureus str. Newman reduction in abundance of anchored SpA by FITC-labeled human IgG fluorescence based assay
Assay data:1 Active, 3 Tested
Inhibition of Staphylococcus aureus Newman recombinant SrtA expressed in Escherichia coli cells using Dabcyl-QALPETGEE-Edans as substrate by fluorimetric analysis
Inhibition of Staphylococcus aureus Newman SrtA assessed as decrease in SpA display at 200 uM by SDS/PAGE immunoblotting assay relative to control
Inhibition of Staphylococcus aureus Newman SrtA assessed as decrease in SpA display by SDS/PAGE immunoblotting assay
Assay data:2 Tested
Inhibition of Staphylococcus aureus Newman SrtA assessed as decrease in SpA display by FITC-IgG-based fluorescence assay
Inhibition of CrtN in Staphylococcus aureus Newman after 12 resistant development passages under 0.15% v/v H2O2 assessed as reduction in appearance of non-pigmented colonies on TSA plates at 10 nM/ml
Inhibition of CrtN in Staphylococcus aureus Newman after 12 resistant development passages under 0.15% v/v H2O2 assessed as reduction in appearance of non-pigmented colonies on TSA plates at 7.5 nM/ml
Inhibition of CrtN in Staphylococcus aureus Newman after 12 resistant development passages under 0.15% v/v H2O2 assessed as reduction in staphyloxanthin levels under IC90 level after 48 hrs by spectrophotometric method-based pigment inhibition assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Staphylococcus aureus Newman 6His-tagged CrtN expressed in Escherichia coli BL21 (DE3)/pET28a::CrtN assessed as reduction in staphyloxanthin levels using diapophytoene as substrate after overnight incubation by spectrophotometric analysis
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Inhibition of CrtN in Staphylococcus aureus Newman assessed as reduction in staphyloxanthin levels after 48 hrs by spectrophotometric method-based pigment inhibition assay
Assay data:42 Active, 100 Activity ≤ 1 µM, 100 Tested
Inhibition of CrtN in Staphylococcus aureus Newman assessed as bacterial death by measuring survival rate measured at 2 hrs interval for 6 hrs by serial dilution method
Assay data:2 Active, 2 Tested
Inhibition of CrtN in Staphylococcus aureus Newman assessed as bacterial death by measuring survival rate measured after 6 hrs by serial dilution method (Rvb = 8.67%)
Inhibition of Staphylococcus aureus Newman 6His-tagged CrtN expressed in Escherichia coli BL21(DE3) assessed as reduction in staphyloxanthin levels using diapophytoene as substrate after overnight incubation by spectrophotometric analysis
Assay data:5 Active, 5 Activity ≤ 1 µM, 5 Tested
Inhibition of wild type Staphylococcus aureus Newman CrtN assessed as 4,4'-diapophytoene formation by HPLC analysis
Assay data:3 Active, 3 Tested
Inhibition of CrtN in Staphylococcus aureus Newman assessed as reduction in staphyloxanthin levels after 48 hrs by spectrophotometric analysis
Assay data:44 Active, 93 Activity ≤ 1 µM, 93 Tested
Inhibition of CrtN in Staphylococcus aureus Newman assessed as H2O2-induced bacterial death by measuring reduction in bacterial survival at 1 uM pretreated for 24 hrs followed by H2O2 addition measured after 30 mins by serial dilution method
Inhibition of Staphylococcus aureus Newman Hi6-tagged CrtN expressed in Escherichia coli BL21(DE3))/pET28a::CrtN assessed as reduction in staphyloxanthin levels using diapophytoene as substrate after overnight incubation
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
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