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Inhibition of Escherichia coli K-12 beta-glucuronidase using PNPG as substrate preincubated for 5 mins followed by substrate addition measured after 30 mins
Assay data:3 Active, 4 Tested
SummaryCompounds, ActivePubMed Citation
Drug metabolism in Escherichia coli K-12 lysates assessed as esterase-mediated drug hydrolysis by measuring apparent first-order rate constant at 200 uM incubated up to 24 hrs by HPLC analysis
Assay data:4 Tested
SummaryPubMed Citation
Drug metabolism in Escherichia coli K-12 lysates assessed as esterase-mediated drug hydrolysis at 200 uM incubated up to 24 hrs by HPLC analysis
Inhibition of sdiA in Escherichia coli K-12 assessed as reduction in protein-mediated transition from microcolonies to mature biofilm at 400 uM incubated for 22 hrs by crystal violet staining assay relative to control
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of Escherichia coli K12 glycerokinase at 25 to 500 uM using glycerol as substrate after 15 mins by Kinase Glo luminescence assay
Inhibition of bocillin-FL binding to Escherichia coli K12 NusA-fused/His6-tagged PBP2 (57 to 615 residues) expressed in Escherichia coli BL21(DE3) preincubated for 30 mins followed by bocillin-FL addition by RT-fluorescence anisotropy method
Assay data:8 Active, 5 Activity ≤ 1 µM, 9 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to Escherichia coli K12 NusA-fused/His6-tagged PBP2 (57 to 615 residues) expressed in Escherichia coli BL21(DE3) assessed as melting temperature at protein to compound ratio of 1:10 measured after 30 mins using SYPRO orange dye by RT-PCR-based fluorescence assay (Rvb = 47 +/- 0.5 degC)
Assay data:1 Tested
Inhibition of Escherichia coli K-12 MBP-fused C-terminal His-tagged MurA (6508 to 7768 residues) using UNAG as substrate preincubated for 10 mins followed by substrate addition measured after 30 mins in presence of PEP by malachite green dye based spectrophotometric method
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to His6-sumo-tagged Escherichia coli K-12 GyrB ATPase domain expressed in Escherichia coli BL21 (DE3) assessed as change in melting temperature at 1 mM by SYPRO orange dye-based RT-PCR method
Inhibition of Serine-tRNA synthetase in Escherichia coli K-12 BW28357 S30 extract assessed as reduction in tRNA aminoacylation at 2.5 uM preincubated for 5 mins followed by Escherichia coli tRNA and 14C-labeled serine addition measured after 5 mins by liquid scintillation counting method
Assay data:1 Active, 2 Tested
Inhibition of Glycyl-tRNA synthetase in Escherichia coli K-12 BW28357 S30 extract assessed as reduction in tRNA aminoacylation at 2.5 uM preincubated for 5 mins followed by Escherichia coli tRNA and 14C-labeled glycine addition measured after 5 mins by liquid scintillation counting method
Inhibition of Aspartate-tRNA synthetase in Escherichia coli K-12 BW28357 S30 extract assessed as reduction in tRNA aminoacylation at 2.5 uM preincubated for 5 mins followed by Escherichia coli tRNA and 14C-labeled aspartic acid addition measured after 5 mins by liquid scintillation counting method
Inhibition of Tyrosyl-tRNA synthetase in Escherichia coli K-12 BW28357 S30 extract assessed as reduction in tRNA aminoacylation at 2.5 uM preincubated for 5 mins followed by Escherichia coli tRNA and 14C-labeled tyrosine addition measured after 5 mins by liquid scintillation counting method relative to control
Inhibition of Leucyl-tRNA synthetase in Escherichia coli K-12 BW28357 S30 extract assessed as reduction in tRNA aminoacylation at 2.5 uM preincubated for 5 mins followed by Escherichia coli tRNA and 14C-labeled leucine addition measured after 5 mins by liquid scintillation counting method relative to control
Inhibition of Isoleucyl-tRNA synthetase in Escherichia coli K-12 BW28357 S30 extract assessed as reduction in tRNA aminoacylation at 2.5 uM preincubated for 5 mins followed by Escherichia coli tRNA and 14C-labeled isoleucine addition measured after 5 mins by liquid scintillation counting method relative to control
Inhibition of Escherichia coli K12 thymidine phosphorylase expressed in Escherichia coli using thymidine as substrate addition measured up to 20 mins by Uv-vis spectrophotometric method
Inhibition of Escherichia coli K12 thymidine phosphorylase expressed in Escherichia coli preincubated for 10 mins followed by substrate addition measured up to 30 mins by spectrophotometric method
Assay data:1 Active, 3 Tested
Inhibition of recombinant Escherichia coli K12 FtsZ GTPase activity by malachite green sodium molybdate assay
Inhibition of recombinant Escherichia coli K12 FtsZ assembly by light-scattering assay
Inhibition of N-terminal His-tagged recombinant Escherichia coli K-12 RNase T exonuclease activity expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL preincubated for 10 mins followed by [gamma-32P]ATP labelled 11-nucleotide ssDNA substrate addition for 40 mins by PAGE based autoradiography
Assay data:2 Active, 9 Tested
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