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Inhibition of Ostrinia furnacalis chitinase-h expressed in Pichia pastoris GS115 using MU-(GlcNAc)2 as substrate incubated for 30 mins by microplate reader analysis
Assay data:12 Active, 5 Activity ≤ 1 µM, 15 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Binding affinity to Ostrinia furnacalis chitinase h catalytic domain overexpressed in Pichia pastoris by isothermal titration calorimetry
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Ostrinia furnacalis chitinase h catalytic domain overexpressed in Pichia pastoris using 4MU-(GlcNAc)2 as substrate after 30 mins by fluorescence based microplate reader analysis
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of Ostrinia furnacalis chitinase h catalytic domain overexpressed in Pichia pastoris at 1 uM using 4MU-(GlcNAc)2 as substrate after 30 mins by fluorescence based microplate reader analysis
Assay data:10 Active, 23 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to Ostrinia furnacalis chitinase h catalytic domain overexpressed in Pichia pastoris cells assessed as association constant by isothermal titration calorimetry
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of Ostrinia furnacalis beta-N-acetylhexosaminidase using pNP-beta-GlcNAc as substrate by dixon plot analysis
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of Ostrinia furnacalis Hex1 expressed in Pichia pastoris using varying levels of 4-MU-GlcNAc as substrate measured after 30 mins by fluorescence assay
Assay data:2 Tested
Inhibition of Ostrinia furnacalis Hex1 expressed in Pichia pastoris at 100 uM using 4-MU-GlcNAc as substrate measured after 30 mins by fluorescence assay relative to control
Assay data:16 Tested
Inhibition of Ostrinia furnacalis Hex1 expressed in Pichia pastoris at 200 uM using varying levels of 4-MU-GlcNAc as substrate measured after 30 mins by fluorescence assay relative to control
Antifeedant activity against fifth-instar larval stage of Ostrinia furnacalis (Asian corn borer) assessed as feeding deterrence index at 500 mg/kg after 24 hr by diet-choice assay
Assay data:10 Tested
SummaryPubMed Citation
Antifeedant activity against fifth-instar larval stage of Ostrinia furnacalis (Asian corn borer) assessed as larval weight gain at 5 mg/kg after 5 days by diet-choice assay (Rvb = 43.3 +/- 20.8 mg)
Assay data:3 Tested
Antifeedant activity against fifth-instar larval stage of Ostrinia furnacalis (Asian corn borer) assessed as larval weight gain at 10 mg/kg after 5 days by diet-choice assay (Rvb = 43.3 +/- 20.8 mg)
Antifeedant activity against fifth-instar larval stage of Ostrinia furnacalis (Asian corn borer) assessed as larval weight gain at 20 mg/kg after 5 days by diet-choice assay (Rvb = 43.3 +/- 20.8 mg)
Insecticidal activity against Ostrinia furnacalis (Asian corn borer) after 24 hr by larvae-dipping method
Summary
Contact toxicity against Ostrinia furnacalis (Asian corn borer) fourth-instar larvae exposed to compound on thoracic tergite region assessed as insect mortality measured 48 hr post compound treatment
Contact toxicity against fourth-instar larval stage of Ostrinia furnacalis (Asian corn borer) assessed as mortality at 25 +/-1 degC applied topically on the thoracic tergite measured per gm of larva after 48 hr
Toxic ratio of RH-5992 LD50 to compound LD50 for fourth-instar larval stage of Ostrinia furnacalis (Asian corn borer) measured after 48 hr
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