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Select item 99083061.

Epoxidized graphene grid for highly efficient high-resolution cryoEM structural analysis

Junso Fujita, Fumiaki Makino, Haruyasu Asahara, Maiko Moriguchi, Shota Kumano, Itsuki Anzai, Jun-ichi Kishikawa, Yoshiharu Matsuura, Takayuki Kato, Keiichi Namba, Tsuyoshi Inoue

Sci Rep. 2023; 13: 2279. Published online 2023 Feb 8. doi: 10.1038/s41598-023-29396-0

PMCID:: PMC9908306

Abstract Article PDF–3.0M

Select item 65123382.

2.2 Å resolution cryo-EM structure of β-galactosidase in complex with a cell-permeant inhibitor

Alberto Bartesaghi, Alan Merk, Soojay Banerjee, Doreen Matthies, Xiongwu Wu, Jacqueline L. S. Milne, Sriram Subramaniam

Science. Author manuscript; available in PMC 2019 May 13.

Published in final edited form as: Science. 2015 Jun 5; 348(6239): 1147–1151. Published online 2015 May 7. doi: 10.1126/science.aab1576

PMCID:: PMC6512338

Abstract Article PDF–1.0M

Select item 41366293.

Structure of β-galactosidase at 3.2-Å resolution obtained by cryo-electron microscopy

Alberto Bartesaghi, Doreen Matthies, Soojay Banerjee, Alan Merk, Sriram Subramaniam

Proc Natl Acad Sci U S A. 2014 Aug 12; 111(32): 11709–11714. Published online 2014 Jul 28. doi: 10.1073/pnas.1402809111

PMCID:: PMC4136629

Abstract Article PDF–1.8M

Select item 39889984.

Molecular Mechanism of Antibody-Mediated Activation of β-galactosidase

Kutti R. Vinothkumar, Greg McMullan, Richard Henderson

Structure. 2014 Apr 8; 22(4): 621–627. doi: 10.1016/j.str.2014.01.011

PMCID:: PMC3988998

Abstract Article

Select item 37374735.

The Role of Salt Bridges, Charge Density, and Subunit Flexibility in Determining Disassembly Routes of Protein Complexes

Zoe Hall, Helena Hernández, Joseph A. Marsh, Sarah A. Teichmann, Carol V. Robinson

Structure. 2013 Aug 6; 21(8): 1325–1337. doi: 10.1016/j.str.2013.06.004

PMCID:: PMC3737473

Abstract Article

Select item 33025966.

Disulfide Bond Formation and Activation of Escherichia coli β-Galactosidase under Oxidizing Conditions

Joaquin Seras-Franzoso, Roman Affentranger, Mario Ferrer-Navarro, Xavier Daura, Antonio Villaverde, Elena García-Fruitós

Appl Environ Microbiol. 2012 Apr; 78(7): 2376–2385. doi: 10.1128/AEM.06923-11

PMCID:: PMC3302596

Abstract Article PDF–1.9M

Select item 31223117.

Insights Into Mutagenesis Using Escherichia coli Chromosomal lacZ Strains That Enable Detection of a Wide Spectrum of Mutational Events

Tracey Seier, Dana R. Padgett, Gal Zilberberg, Vincent A. Sutera, Jr., Noor Toha, Susan T. Lovett

Genetics. 2011 Jun; 188(2): 247–262. doi: 10.1534/genetics.111.127746

PMCID:: PMC3122311

Abstract Article PDF–1.1M

Select item 27744388.

Direct and indirect roles of His-418 in metal binding and in the activity of β-galactosidase (E. coli)

Douglas H Juers, Beatrice Rob, Megan L Dugdale, Nastaron Rahimzadeh, Clarence Giang, Michelle Lee, Brian W Matthews, Reuben E Huber

Protein Sci. 2009 Jun; 18(6): 1281–1292. Published online 2009 Apr 16. doi: 10.1002/pro.140

PMCID:: PMC2774438

Abstract Article PDF–696K

Select item 27318969.

Conditional gene silencing of multiple genes with antisense RNAs and generation of a mutator strain of Escherichia coli

Nobutaka Nakashima, Tomohiro Tamura

Nucleic Acids Res. 2009 Aug; 37(15): e103. Published online 2009 Jun 10. doi: 10.1093/nar/gkp498

PMCID:: PMC2731896

Abstract Article PDF–385K

Select item 272675310.

Structure-Reactivity Relationships for β-Galactosidase (Escherichia coli, lac Z): A Second Derivative Effect on β_nuc for Addition of Alkyl Alcohols to an Oxocarbenium Ion Reaction Intermediate

John P. Richard, Christina K. Heo, Maria M. Toteva

J Phys Org Chem. Author manuscript; available in PMC 2009 Aug 13.

Published in final edited form as: J Phys Org Chem. 2008 Jul 1; 21(7-8): 531–537. doi: 10.1002/poc.1302

PMCID:: PMC2726753

Abstract Article PDF–998K

Select item 250434011.

Quantitative approaches to the study of bistability in the lac operon of Escherichia coli

Moisés Santillán, Michael C. Mackey

J R Soc Interface. 2008 Aug 6; 5(Suppl 1): S29–S39. Published online 2008 Apr 15. doi: 10.1098/rsif.2008.0086.focus

PMCID:: PMC2504340

Abstract Article PDF–289K

Select item 222373612.

Substitutions at Auxiliary Operator O3 Enhance Repression by Nitrate-Responsive Regulator NarL at Synthetic lac Control Regions in Escherichia coli K-12

Valley Stewart, Peggy J. Bledsoe

J Bacteriol. 2008 Jan; 190(1): 428–433. Published online 2007 Oct 26. doi: 10.1128/JB.01431-07

PMCID:: PMC2223736

Abstract Article PDF–112K

Select item 217293413.

Modeling network dynamics: the lac operon, a case study

José M.G. Vilar, Călin C. Guet, Stanislas Leibler

J Cell Biol. 2003 May 12; 161(3): 471–476. doi: 10.1083/jcb.200301125

PMCID:: PMC2172934

Abstract Article PDF–195K

Select item 214471314.

High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation.

D. H. Juers, R. H. Jacobson, D. Wigley, X. J. Zhang, R. E. Huber, D. E. Tronrud, B. W. Matthews

Protein Sci. 2000 Sep; 9(9): 1685–1699. doi: 10.1110/ps.9.9.1685

PMCID:: PMC2144713

Summary PDF–4.1M

Select item 201472215.

Site-saturation Mutagenesis is more Efficient than DNA Shuffling for the Directed Evolution of β-Fucosidase from β-Galactosidase

Monal R. Parikh, Ichiro Matsumura

J Mol Biol. Author manuscript; available in PMC 2007 Oct 10.

Published in final edited form as: J Mol Biol. 2005 Sep 23; 352(3): 621–628. doi: 10.1016/j.jmb.2005.07.020

PMCID:: PMC2014722

Abstract Article PDF–930K

Select item 189937016.

Amplification of lac Cannot Account for Adaptive Mutation to Lac⁺ in Escherichia coli

Jeffrey D. Stumpf, Anthony R. Poteete, Patricia L. Foster

J Bacteriol. 2007 Mar; 189(6): 2291–2299. Published online 2007 Jan 5. doi: 10.1128/JB.01706-06

PMCID:: PMC1899370

Abstract Article PDF–361K

Select item 189482017.

The Effect of Stochasticity on the Lac Operon: An Evolutionary Perspective

Milan van Hoek, Paulien Hogeweg

PLoS Comput Biol. 2007 Jun; 3(6): e111. Published online 2007 Jun 22. doi: 10.1371/journal.pcbi.0030111

PMCID:: PMC1894820

Abstract Article PDF–1.4M

Select item 168148118.

Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110

Koji Hayashi, Naoki Morooka, Yoshihiro Yamamoto, Katsutoshi Fujita, Katsumi Isono, Sunju Choi, Eiichi Ohtsubo, Tomoya Baba, Barry L Wanner, Hirotada Mori, Takashi Horiuchi

Mol Syst Biol. 2006; 2: 2006.0007. Published online 2006 Feb 21. doi: 10.1038/msb4100049