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Items: 1 to 20 of 93

1.
Characterization of Cwc2, U6 snRNA, and Prp8 interactions destabilized by Prp16 ATPase at the transition between the first and second steps of splicing
Jadwiga Meissner, Katarzyna Eysmont, Katarzyna Matylla-Kulińska, Maria M. Konarska
RNA. 2024 Sep; 30(9): 1199–1212. doi: 10.1261/rna.079886.123
PMCID:
PMC11331412
AbstractArticlePDF–6.7M
2.
Identification of transient intermediates during spliceosome activation by single molecule fluorescence microscopy
Xingyang Fu, Harpreet Kaur, Margaret L. Rodgers, Eric J. Montemayor, Samuel E. Butcher, Aaron A. Hoskins
Proc Natl Acad Sci U S A. 2022 Nov 29; 119(48): e2206815119. Published online 2022 Nov 23. doi: 10.1073/pnas.2206815119
PMCID:
PMC9860250
AbstractArticlePDF–4.0M
3.
Saccharomyces cerevisiae Ecm2 modulates the catalytic steps of pre-mRNA splicing
Clarisse van der Feltz, Brandon Nikolai, Charles Schneider, Joshua C. Paulson, Xingyang Fu, Aaron A. Hoskins
RNA. 2021 May; 27(5): 591–603. doi: 10.1261/rna.077727.120
PMCID:
PMC8051269
AbstractArticlePDF–6.5M
4.
Termination of pre-mRNA splicing requires that the ATPase and RNA unwindase Prp43p acts on the catalytic snRNA U6
Rebecca Toroney, Klaus H. Nielsen, Jonathan P. Staley
Genes Dev. 2019 Nov 1; 33(21-22): 1555–1574. doi: 10.1101/gad.328294.119
PMCID:
PMC6824469
AbstractArticlePDF–5.5M
5.
Spt5 modulates cotranscriptional spliceosome assembly in Saccharomyces cerevisiae
Isabella E. Maudlin, Jean D. Beggs
RNA. 2019 Oct; 25(10): 1298–1310. doi: 10.1261/rna.070425.119
PMCID:
PMC6800482
AbstractArticlePDF–1.1M
6.
An Allosteric Network for Spliceosome Activation Revealed by High-Throughput Suppressor Analysis in Saccharomyces cerevisiae
David A. Brow
Genetics. 2019 May; 212(1): 111–124. Published online 2019 Mar 21. doi: 10.1534/genetics.119.301922
PMCID:
PMC6499515
AbstractArticlePDF–2.4M
7.
Multiple RNA–RNA tertiary interactions are dispensable for formation of a functional U2/U6 RNA catalytic core in the spliceosome
Penghui Bao, Kum-Loong Boon, Cindy L Will, Klaus Hartmuth, Reinhard Lührmann
Nucleic Acids Res. 2018 Dec 14; 46(22): 12126–12138. Published online 2018 Oct 18. doi: 10.1093/nar/gky966
PMCID:
PMC6294511
AbstractArticlePDF–4.7M
8.
Prespliceosome structure provides insights into spliceosome assembly and regulation
Clemens Plaschka, Pei-Chun Lin, Clément Charenton, Kiyoshi Nagai
Nature. Author manuscript; available in PMC 2019 Jan 11.
Published in final edited form as: Nature. 2018 Jul; 559(7714): 419–422. Published online 2018 Jul 11. doi: 10.1038/s41586-018-0323-8
PMCID:
PMC6141012
AbstractArticlePDF–6.5M
9.
Defining essential elements and genetic interactions of the yeast Lsm2–8 ring and demonstration that essentiality of Lsm2–8 is bypassed via overexpression of U6 snRNA or the U6 snRNP subunit Prp24
Allen J. Roth, Stewart Shuman, Beate Schwer
RNA. 2018 Jun; 24(6): 853–864. doi: 10.1261/rna.066175.118
PMCID:
PMC5959253
AbstractArticlePDF–7.4M
10.
Architecture of the U6 snRNP reveals specific recognition of 3′-end processed U6 snRNA
Eric J. Montemayor, Allison L. Didychuk, Allyson D. Yake, Gurnimrat K. Sidhu, David A. Brow, Samuel E. Butcher
Nat Commun. 2018; 9: 1749. Published online 2018 May 1. doi: 10.1038/s41467-018-04145-4
PMCID:
PMC5931518
AbstractArticlePDF–2.1M
11.
Structure of the yeast spliceosomal post-catalytic P complex
Shiheng Liu, Xueni Li, Lingdi Zhang, Jiansen Jiang, Ryan C. Hill, Yanxiang Cui, Kirk C. Hansen, Z. Hong Zhou, Rui Zhao
Science. Author manuscript; available in PMC 2018 Feb 27.
Published in final edited form as: Science. 2017 Dec 8; 358(6368): 1278–1283. Published online 2017 Nov 16. doi: 10.1126/science.aar3462
PMCID:
PMC5828012
AbstractArticlePDF–976K
12.
The RES complex is required for efficient transformation of the precatalytic B spliceosome into an activated Bact complex
Penghui Bao, Cindy L. Will, Henning Urlaub, Kum-Loong Boon, Reinhard Lührmann
Genes Dev. 2017 Dec 1; 31(23-24): 2416–2429. doi: 10.1101/gad.308163.117
PMCID:
PMC5795787
AbstractArticlePDF–7.6M
13.
Yeast Prp2 liberates the 5′ splice site and the branch site adenosine for catalysis of pre-mRNA splicing
Penghui Bao, Claudia Höbartner, Klaus Hartmuth, Reinhard Lührmann
RNA. 2017 Dec; 23(12): 1770–1779. doi: 10.1261/rna.063115.117
PMCID:
PMC5688998
AbstractArticlePDF–5.4M
14.
Usb1 controls U6 snRNP assembly through evolutionarily divergent cyclic phosphodiesterase activities
Allison L. Didychuk, Eric J. Montemayor, Tucker J. Carrocci, Andrew T. DeLaitsch, Stefani E. Lucarelli, William M. Westler, David A. Brow, Aaron A. Hoskins, Samuel E. Butcher
Nat Commun. 2017; 8: 497. Published online 2017 Sep 8. doi: 10.1038/s41467-017-00484-w
PMCID:
PMC5591277
AbstractArticlePDF–2.3M
15.
Structure of a spliceosome remodelled for exon ligation
Sebastian M. Fica, Chris Oubridge, Wojciech P. Galej, Max E. Wilkinson, Xiao-Chen Bai, Andrew J. Newman, Kiyoshi Nagai
Nature. Author manuscript; available in PMC 2017 Jul 11.
Published in final edited form as: Nature. 2017 Feb 16; 542(7641): 377–380. Published online 2017 Jan 11. doi: 10.1038/nature21078
PMCID:
PMC5321579
AbstractArticlePDF–5.6M
16.
A multi-step model for facilitated unwinding of the yeast U4/U6 RNA duplex
Margaret L. Rodgers, Allison L. Didychuk, Samuel E. Butcher, David A. Brow, Aaron A. Hoskins
Nucleic Acids Res. 2016 Dec 15; 44(22): 10912–10928. Published online 2016 Aug 2. doi: 10.1093/nar/gkw686
PMCID:
PMC5159527
AbstractArticlePDF–2.6M
17.
Decoding the principles underlying the frequency of association with nucleoli for RNA polymerase III–transcribed genes in budding yeast
Praveen Belagal, Christophe Normand, Ashutosh Shukla, Renjie Wang, Isabelle Léger-Silvestre, Christophe Dez, Purnima Bhargava, Olivier Gadal
Mol Biol Cell. 2016 Oct 15; 27(20): 3164–3177. doi: 10.1091/mbc.E16-03-0145
PMCID:
PMC5063623
AbstractArticlePDF–4.4M
18.
Single molecule analysis reveals reversible and irreversible steps during spliceosome activation
Aaron A Hoskins, Margaret L Rodgers, Larry J Friedman, Jeff Gelles, Melissa J Moore
eLife. 2016; 5: e14166. Published online 2016 May 31. doi: 10.7554/eLife.14166
PMCID:
PMC4922858
AbstractArticlePDF–2.9M
19.
Structural analysis of multi-helical RNAs by NMR-SAXS/WAXS: Application to the U4/U6 di-snRNA
Gabriel Cornilescu, Allison L. Didychuk, Margaret L. Rodgers, Lauren A. Michael, Jordan E. Burke, Eric J. Montemayor, Aaron A. Hoskins, Samuel E. Butcher
J Mol Biol. Author manuscript; available in PMC 2017 Feb 27.
Published in final edited form as: J Mol Biol. 2016 Feb 27; 428(5 Pt A): 777–789. Published online 2015 Dec 2. doi: 10.1016/j.jmb.2015.11.026
PMCID:
PMC4790120
AbstractArticlePDF–758K
20.
CryoEM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution
Thi Hoang Duong Nguyen, Wojciech P. Galej, Xiao-chen Bai, Chris Oubridge, Andrew J. Newman, Sjors H. W. Scheres, Kiyoshi Nagai
Nature. Author manuscript; available in PMC 2016 Aug 18.
Published in final edited form as: Nature. 2016 Feb 18; 530(7590): 298–302. Published online 2016 Feb 1. doi: 10.1038/nature16940
PMCID:
PMC4762201
AbstractArticlePDF–6.1M

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