LOCUS NP_015185 506 aa linear PLN 17-DEC-2024
DEFINITION putative mitogen-activated protein kinase kinase MKK2
[Saccharomyces cerevisiae S288C].
ACCESSION NP_015185
VERSION NP_015185.1
DBLINK BioProject: PRJNA128
DBSOURCE REFSEQ: accession NM_001183954.1
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (residues 1 to 506)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (residues 1 to 506)
AUTHORS Bussey,H., Storms,R.K., Ahmed,A., Albermann,K., Allen,E.,
Ansorge,W., Araujo,R., Aparicio,A., Barrell,B., Badcock,K.,
Benes,V., Botstein,D., Bowman,S., Bruckner,M., Carpenter,J.,
Cherry,J.M., Chung,E., Churcher,C., Coster,F., Davis,K.,
Davis,R.W., Dietrich,F.S., Delius,H., DiPaolo,T., Hani,J. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI
JOURNAL Nature 387 (6632 SUPPL), 103-105 (1997)
PUBMED 9169875
REFERENCE 3 (residues 1 to 506)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 4 (residues 1 to 506)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (17-DEC-2024) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (residues 1 to 506)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (16-JAN-2015) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (residues 1 to 506)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 7 (residues 1 to 506)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to DAA11294.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
Method: conceptual translation.
FEATURES Location/Qualifiers
source 1..506
/organism="Saccharomyces cerevisiae S288C"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="XVI"
Protein 1..506
/product="putative mitogen-activated protein kinase kinase
MKK2"
/EC_number="2.7.12.2"
/calculated_mol_wt=56627
Region 212..499
/region_name="PKc_Pek1_like"
/note="Catalytic domain of fungal Pek1-like
dual-specificity Mitogen-Activated Protein Kinase Kinases;
cd06621"
/db_xref="CDD:270793"
Site order(220..224,228,241,243,274,292..295,298..299,302,342,
344..347,349,359..360,363,375..378,380,407,416)
/site_type="active"
/db_xref="CDD:270793"
Site order(220..224,226,228,241,243,274,292..295,298..299,
301..302,344,346..347,349,360)
/site_type="other"
/note="ATP binding site [chemical binding]"
/db_xref="CDD:270793"
Site order(223..224,342,344..346,363,375..378,380,407,416)
/site_type="other"
/note="polypeptide substrate binding site [polypeptide
binding]"
/db_xref="CDD:270793"
Site 359..380
/site_type="other"
/note="activation loop (A-loop)"
/db_xref="CDD:270793"
CDS 1..506
/gene="MKK2"
/locus_tag="YPL140C"
/gene_synonym="LPI6; SSP33"
/coded_by="NM_001183954.1:1..1521"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12361575]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:12361575]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12361575]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12361575]"
/experiment="EXISTENCE:genetic interaction:GO:0000196 cell
integrity MAPK cascade [PMID:8386320]"
/experiment="EXISTENCE:genetic interaction:GO:0000425
pexophagy [PMID:20385774]"
/experiment="EXISTENCE:genetic interaction:GO:0060237
regulation of fungal-type cell wall organization
[PMID:8386320]"
/experiment="EXISTENCE:mutant phenotype:GO:0007165 signal
transduction [PMID:8386320]"
/note="MAPKK involved in the protein kinase C signaling
pathway; involved in control of cell integrity; upon
activation by Bck1p phosphorylates downstream target,
Slt2p; functionally redundant with Mkk1p; MKK2 has a
paralog, MKK1, that arose from the whole genome
duplication"
/db_xref="GeneID:855963"
/db_xref="SGD:S000006061"
ORIGIN
1 masmfrppes nrshqktpkl tlpvnlvqna kstndgqhln rspyssvnes pysnnstsat
61 sttssmasns tllynrsstt tiknrpvppp lpplvltqkk dgieyrvagd sqlserfsnl
121 hvditykell ssapistkls nidttfikkd ldtpegedsy pstllsaydf sssgsnsapl
181 sanniiscsn liqgkdvdql eeeawrfghl kdeittlgil gegaggsvak crlkngkkvf
241 alktintmnt dpeyqkqifr elqfnksfks dyivqyygmf tdeqsssiyi ameymggksl
301 eatyknllkr ggriservig kiaesvlrgl sylherkvih rdikpqnill nekgeiklcd
361 fgvsgeavns lamtftgtsf ymaperiqgq pysvtcdvws lgltllevag grfpfesdki
421 tqnvapiell tmiltfspql kdepeldisw sktfrsfidy clkkdarerp sprqmlkhpw
481 ivgqmkkkvn merfvkkcwe kekdgi
//