LOCUS NP_012273 409 aa linear PLN 17-DEC-2024
DEFINITION DNA primase subunit PRI1 [Saccharomyces cerevisiae S288C].
ACCESSION NP_012273
VERSION NP_012273.1
DBLINK BioProject: PRJNA128
DBSOURCE REFSEQ: accession NM_001179530.1
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (residues 1 to 409)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (residues 1 to 409)
AUTHORS Churcher,C., Bowman,S., Badcock,K., Bankier,A., Brown,D.,
Chillingworth,T., Connor,R., Devlin,K., Gentles,S., Hamlin,N.,
Harris,D., Horsnell,T., Hunt,S., Jagels,K., Jones,M., Lye,G.,
Moule,S., Odell,C., Pearson,D., Rajandream,M., Rice,P., Rowley,N.,
Skelton,J., Smith,V., Barrell,B. et al.
TITLE The nucleotide sequence of Saccharomyces cerevisiae chromosome IX
JOURNAL Nature 387 (6632 SUPPL), 84-87 (1997)
PUBMED 9169870
REFERENCE 3 (residues 1 to 409)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 4 (residues 1 to 409)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (17-DEC-2024) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (residues 1 to 409)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (04-MAY-2012) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Protein update by submitter
REFERENCE 6 (residues 1 to 409)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 7 (residues 1 to 409)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (14-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to DAA08554.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-4-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
Method: conceptual translation.
FEATURES Location/Qualifiers
source 1..409
/organism="Saccharomyces cerevisiae S288C"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="IX"
Protein 1..409
/product="DNA primase subunit PRI1"
/calculated_mol_wt=47559
Region 13..362
/region_name="primase_sml"
/note="DNA primase, eukaryotic-type, small subunit,
putative; TIGR00335"
/db_xref="CDD:273020"
Site order(111,113,115,162,165..166,168,314,323..324,326,332)
/site_type="other"
/note="nucleotide binding site [chemical binding]"
/db_xref="CDD:240130"
Site order(130,133)
/site_type="other"
/note="zinc binding site [ion binding]"
/db_xref="CDD:240130"
Site order(150..152,190,195,210,213)
/site_type="other"
/note="subunit interface [polypeptide binding]"
/db_xref="CDD:240130"
CDS 1..409
/gene="PRI1"
/locus_tag="YIR008C"
/coded_by="NM_001179530.1:1..1230"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:20404922]"
/experiment="EXISTENCE:direct assay:GO:0003896 DNA primase
activity [PMID:3888995|PMID:3061469|PMID:2644256]"
/experiment="EXISTENCE:direct assay:GO:0005658 alpha DNA
polymerase:primase complex [PMID:3888995|PMID:22593576]"
/experiment="EXISTENCE:direct assay:GO:0006269 DNA
replication, synthesis of primer [PMID:2644256]"
/experiment="EXISTENCE:direct assay:GO:0043596 nuclear
replication fork [PMID:16103218]"
/experiment="EXISTENCE:genetic interaction:GO:0003896 DNA
primase activity [PMID:8436268]"
/experiment="EXISTENCE:mutant phenotype:GO:0003896 DNA
primase activity [PMID:8436268]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:2023935]"
/note="Subunit of DNA primase; DNA primase is required for
DNA synthesis and double-strand break repair"
/db_xref="GeneID:854825"
/db_xref="SGD:S000001447"
ORIGIN
1 mtnsvktngp sssdmeyyyk slypfkhifn wlnhspkpsr dminrefama frsgaykryn
61 sfnsvqdfka qiekanpdrf eigaiynkpp rerdtllkse lkalekelvf didmddydaf
121 rtccsgaqvc skcwkfisla mkitntalre dfgykdfiwv fsgrrgahcw vsdkraralt
181 dvqrrnvldy vnvirdrntd krlalkrpyh phlarsleql kpffvsimle eqnpweddqh
241 aiqtllpaly dkqlidslkk ywldnprrss kekwndidqi atslfkgpkq dshiiklrec
301 kedlvlmtly pkldvevtkq tihllkapfc ihpatgnvcv pidesfapek apklidlqte
361 meknndvslt alqpfinqfq ayvssllkne lgsvkrered ddepasldf
//