U.S. flag

An official website of the United States government

aspartate transaminase AAT2 [Saccharomyces cerevisiae S288C]

NCBI Reference Sequence: NP_013127.2

Identical Proteins FASTA Graphics 

LOCUS       NP_013127                418 aa            linear   PLN 17-DEC-2024
DEFINITION  aspartate transaminase AAT2 [Saccharomyces cerevisiae S288C].
ACCESSION   NP_013127
VERSION     NP_013127.2
DBLINK      BioProject: PRJNA128
DBSOURCE    REFSEQ: accession NM_001181914.1
KEYWORDS    RefSeq.
SOURCE      Saccharomyces cerevisiae S288C
  ORGANISM  Saccharomyces cerevisiae S288C
            Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
            Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
            Saccharomyces.
REFERENCE   1  (residues 1 to 418)
  AUTHORS   Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
            Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
            Weng,S. and Cherry,J.M.
  TITLE     New data and collaborations at the Saccharomyces Genome Database:
            updated reference genome, alleles, and the Alliance of Genome
            Resources
  JOURNAL   Genetics 220 (4) (2022)
   PUBMED   34897464
REFERENCE   2  (residues 1 to 418)
  AUTHORS   Johnston,M., Hillier,L., Riles,L., Albermann,K., Andre,B.,
            Ansorge,W., Benes,V., Bruckner,M., Delius,H., Dubois,E.,
            Dusterhoft,A., Entian,K.D., Floeth,M., Goffeau,A., Hebling,U.,
            Heumann,K., Heuss-Neitzel,D., Hilbert,H., Hilger,F., Kleine,K.,
            Kotter,P., Louis,E.J., Messenguy,F., Mewes,H.W., Hoheisel,J.D. et
            al.
  TITLE     The nucleotide sequence of Saccharomyces cerevisiae chromosome XII
  JOURNAL   Nature 387 (6632 SUPPL), 87-90 (1997)
   PUBMED   9169871
REFERENCE   3  (residues 1 to 418)
  AUTHORS   Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
            Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
            Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
            Oliver,S.G.
  TITLE     Life with 6000 genes
  JOURNAL   Science 274 (5287), 546 (1996)
   PUBMED   8849441
REFERENCE   4  (residues 1 to 418)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2024) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   5  (residues 1 to 418)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2015) Department of Genetics, Stanford
            University, Stanford, CA, USA
  REMARK    Protein update by submitter
REFERENCE   6  (residues 1 to 418)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2013) Department of Genetics, Stanford
            University, Stanford, CA, USA
  REMARK    Protein update by submitter
REFERENCE   7  (residues 1 to 418)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2012) Department of Genetics, Stanford
            University, Stanford, CA, USA
  REMARK    Protein update by submitter
REFERENCE   8  (residues 1 to 418)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2011) Department of Genetics, Stanford
            University, Stanford, CA, USA
  REMARK    Sequence update by submitter
REFERENCE   9  (residues 1 to 418)
  CONSRTM   Saccharomyces Genome Database
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2009) Department of Genetics, Stanford
            University, Stanford, CA, USA
COMMENT     REVIEWED REFSEQ: This record has been curated by SGD. The reference
            sequence is identical to DAA09345.
            
            On Oct 2, 2003 this sequence version replaced NP_013127.1.
            
            ##Genome-Annotation-Data-START##
            Annotation Provider :: SGD
            Annotation Status   :: Full Annotation
            Annotation Version  :: R64-4-1
            URL                 :: http://www.yeastgenome.org/
            ##Genome-Annotation-Data-END##
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Saccharomyces cerevisiae S288C"
                     /strain="S288C"
                     /db_xref="taxon:559292"
                     /chromosome="XII"
     Protein         1..418
                     /product="aspartate transaminase AAT2"
                     /EC_number="2.6.1.1"
                     /calculated_mol_wt=45927
     Region          2..410
                     /region_name="AAT_I"
                     /note="Aspartate aminotransferase (AAT) superfamily (fold
                     type I) of pyridoxal phosphate (PLP)-dependent enzymes.
                     PLP combines with an alpha-amino acid to form a compound
                     called a Schiff base or aldimine intermediate, which
                     depending on the reaction, is the...; cl18945"
                     /db_xref="CDD:450240"
     Site            order(107..109,135,188,219,252,254..255,263)
                     /site_type="other"
                     /note="pyridoxal 5'-phosphate binding site [chemical
                     binding]"
                     /db_xref="CDD:99734"
     Site            order(110,145,212,261..263,303,306)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:99734"
     Site            255
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:99734"
     CDS             1..418
                     /gene="AAT2"
                     /locus_tag="YLR027C"
                     /gene_synonym="ASP5"
                     /coded_by="NM_001181914.1:1..1257"
                     /experiment="EXISTENCE:direct assay:GO:0004069
                     L-aspartate:2-oxoglutarate aminotransferase activity
                     [PMID:1859361]"
                     /experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
                     [PMID:9288922]"
                     /experiment="EXISTENCE:direct assay:GO:0005829 cytosol
                     [PMID:9288922]"
                     /experiment="EXISTENCE:direct assay:GO:0043023 ribosomal
                     large subunit binding [PMID:35621265]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0004069
                     L-aspartate:2-oxoglutarate aminotransferase activity
                     [PMID:9288922]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0005777
                     peroxisome [PMID:9288922]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0006532
                     aspartate biosynthetic process
                     [PMID:15780654|PMID:9288922]"
                     /experiment="EXISTENCE:mutant phenotype:GO:0032938
                     negative regulation of translation in response to
                     oxidative stress [PMID:35621265]"
                     /note="Cytosolic aspartate aminotransferase involved in
                     the metabolism of nitrogen and amino acids such as
                     aspartate; localizes to and functions in peroxisomes in
                     oleate-grown cells; moonlighting role as a
                     ribosome-associated protein that modulates translation in
                     response to stress"
                     /db_xref="GeneID:850714"
                     /db_xref="SGD:S000004017"
ORIGIN      
        1 msatlfnnie llppdalfgi kqrygqdqra tkvdlgigay rddngkpwvl psvkaaekli
       61 hndssynhey lgitglpslt snaakiifgt qsdafqedrv isvqslsgtg alhisakffs
      121 kffpdklvyl skptwanhma ifenqglkta typywanetk sldlngflna iqkapegsif
      181 vlhscahnpt gldptseqwv qivdaiaskn hialfdtayq gfatgdldkd ayavrlgvek
      241 lstvspvfvc qsfaknagmy gervgcfhla ltkqaqnkti kpavtsqlak iirsevsnpp
      301 aygakivakl letpelteqw hkdmvtmssr itkmrhalrd hlvklgtpgn wdhivnqcgm
      361 fsftgltpqm vkrleethav ylvasgrasi aglnqgnvey vakaidevvr fytieakl
//
Feature
Display: FASTA GenBank Help
Details

Supplemental Content

Change region shown

Customize view

Protein 3D Structure

Reference sequence information

  • RefSeq mRNA
    See reference mRNA sequence for the AAT2 gene (NM_001181914.1).

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...
External link. Please review our privacy policy.