LOCUS TRUB_SHIF8 314 aa linear BCT 27-NOV-2024
DEFINITION RecName: Full=tRNA pseudouridine synthase B; AltName: Full=tRNA
pseudouridine(55) synthase; Short=Psi55 synthase; AltName:
Full=tRNA pseudouridylate synthase; AltName: Full=tRNA-uridine
isomerase.
ACCESSION Q0T0B5
VERSION Q0T0B5.1
DBSOURCE UniProtKB: locus TRUB_SHIF8, accession Q0T0B5;
class: standard.
created: May 20, 2008.
sequence updated: Sep 5, 2006.
annotation updated: Nov 27, 2024.
xrefs: CP000266.1, ABF05250.1, WP_000089698.1
xrefs (non-sequence databases): AlphaFoldDB:Q0T0B5, SMR:Q0T0B5,
GeneID:75173340, KEGG:sfv:SFV_3196, HOGENOM:CLU_032087_0_3_6,
Proteomes:UP000000659, GO:0003723, GO:0160148, GO:1990481,
GO:0031119, CDD:cd02573, CDD:cd21152, FunFam:2.30.130.10:FF:000004,
FunFam:3.30.2350.10:FF:000003, Gene3D:3.30.2350.10,
Gene3D:2.30.130.10, HAMAP:MF_01080, InterPro:IPR020103,
InterPro:IPR002501, InterPro:IPR015947, InterPro:IPR036974,
InterPro:IPR014780, InterPro:IPR015240, InterPro:IPR032819,
NCBIfam:TIGR00431, PANTHER:PTHR13767:SF2, PANTHER:PTHR13767,
Pfam:PF09157, Pfam:PF16198, Pfam:PF01509, SUPFAM:SSF55120,
SUPFAM:SSF88697
KEYWORDS Isomerase; tRNA processing.
SOURCE Shigella flexneri 5 str. 8401
ORGANISM Shigella flexneri 5 str. 8401
Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE 1 (residues 1 to 314)
AUTHORS Nie,H., Yang,F., Zhang,X., Yang,J., Chen,L., Wang,J., Xiong,Z.,
Peng,J., Sun,L., Dong,J., Xue,Y., Xu,X., Chen,S., Yao,Z., Shen,Y.
and Jin,Q.
TITLE Complete genome sequence of Shigella flexneri 5b and comparison
with Shigella flexneri 2a
JOURNAL BMC Genomics 7, 173 (2006)
PUBMED 16822325
REMARK NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].;
STRAIN=8401
Publication Status: Online-Only
COMMENT [FUNCTION] Responsible for synthesis of pseudouridine from
uracil-55 in the psi GC loop of transfer RNAs.
{ECO:0000255|HAMAP-Rule:MF_01080}.
[CATALYTIC ACTIVITY] Reaction=uridine(55) in tRNA =
pseudouridine(55) in tRNA; Xref=Rhea:RHEA:42532,
Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102, ChEBI:CHEBI:65314,
ChEBI:CHEBI:65315; EC=5.4.99.25;
Evidence={ECO:0000255|HAMAP-Rule:MF_01080}.
[SIMILARITY] Belongs to the pseudouridine synthase TruB family.
Type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_01080}.
FEATURES Location/Qualifiers
source 1..314
/organism="Shigella flexneri 5 str. 8401"
/db_xref="taxon:373384"
gene 1..314
/gene="truB"
/locus_tag="SFV_3196"
Protein 1..314
/product="tRNA pseudouridine synthase B"
/EC_number="5.4.99.25"
/note="tRNA pseudouridine(55) synthase; tRNA
pseudouridylate synthase; tRNA-uridine isomerase; Psi55
synthase"
/UniProtKB_evidence="Inferred from homology"
Region 1..314
/region_name="Mature chain"
/note="tRNA pseudouridine synthase B. /id=PRO_1000084683."
Region 13..311
/region_name="TruB"
/note="tRNA U55 pseudouridine synthase TruB, may also work
on U342 of tmRNA [Translation, ribosomal structure and
biogenesis]; COG0130"
/db_xref="CDD:439900"
Site 48
/site_type="active"
/note="Nucleophile.
/evidence=ECO:0000255|HAMAP-Rule:MF_01080."
ORIGIN
1 msrprrrgrd ingvllldkp qgmssndalq kvkriynanr aghtgaldpl atgmlpiclg
61 eatkfsqyll dsdkryrvia rlgqrtdtsd adgqiveerp vtfsaeqlaa aldtfrgdie
121 qipsmysalk yqgkklyeya rqgievprea rpitvyellf irhegnelel eihcskgtyi
181 rtiiddlgek lgcgahviyl rrlavskypv ermvtlehlr elveqaeqqd ipaaelldpl
241 lmpmdspasd ypvvnlplts svyfkngnpv rtsgaplegl vrvtegengk figmgeidde
301 grvaprrlvv eypa
//