LOCUS TOXN_BACTK 194 aa linear BCT 27-NOV-2024
DEFINITION RecName: Full=Endoribonuclease ToxN; AltName: Full=Toxin ToxN.
ACCESSION Q3YN09
VERSION Q3YN09.1
DBSOURCE UniProtKB: locus TOXN_BACTK, accession Q3YN09;
class: standard.
plasmid:pAW63
created: Apr 29, 2015.
sequence updated: Sep 27, 2005.
annotation updated: Nov 27, 2024.
xrefs: DQ025752.1, AAZ06636.1, WP_000182254.1, 4ATO_A
xrefs (non-sequence databases): PDBsum:4ATO, AlphaFoldDB:Q3YN09,
SMR:Q3YN09, EvolutionaryTrace:Q3YN09, GO:0003723, GO:0004521,
GO:0006276, FunFam:3.10.129.130:FF:000001, Gene3D:3.10.129.130,
InterPro:IPR025911, InterPro:IPR053735, Pfam:PF13958
KEYWORDS 3D-structure; Coiled coil; Endonuclease; Hydrolase; Nuclease;
Plasmid; RNA-binding; Toxin-antitoxin system.
SOURCE Bacillus thuringiensis serovar kurstaki
ORGANISM Bacillus thuringiensis serovar kurstaki
Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
Bacillus; Bacillus cereus group.
REFERENCE 1 (residues 1 to 194)
AUTHORS Van der Auwera,G.A., Andrup,L. and Mahillon,J.
TITLE Conjugative plasmid pAW63 brings new insights into the genesis of
the Bacillus anthracis virulence plasmid pXO2 and of the Bacillus
thuringiensis plasmid pBT9727
JOURNAL BMC Genomics 6, 103 (2005)
PUBMED 16042811
REMARK NUCLEOTIDE SEQUENCE [GENOMIC DNA].;
STRAIN=ATCC 35866 / NRRL B-4488 / HD-73; PLASMID=pAW63
Publication Status: Online-Only
REFERENCE 2 (residues 1 to 194)
AUTHORS Fineran,P.C., Blower,T.R., Foulds,I.J., Humphreys,D.P., Lilley,K.S.
and Salmond,G.P.
TITLE The phage abortive infection system, ToxIN, functions as a
protein-RNA toxin-antitoxin pair
JOURNAL Proc Natl Acad Sci U S A 106 (3), 894-899 (2009)
PUBMED 19124776
REMARK FUNCTION, AND EXPRESSION IN E.COLI.;
STRAIN=ATCC 35866 / NRRL B-4488 / HD-73; PLASMID=pAW63
REFERENCE 3 (residues 1 to 194)
AUTHORS Short,F.L., Pei,X.Y., Blower,T.R., Ong,S.L., Fineran,P.C.,
Luisi,B.F. and Salmond,G.P.
TITLE Selectivity and self-assembly in the control of a bacterial toxin
by an antitoxic noncoding RNA pseudoknot
JOURNAL Proc Natl Acad Sci U S A 110 (3), E241-E249 (2013)
PUBMED 23267117
REMARK X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH ANTITOXIN
TOXI, FUNCTION, SUBUNIT, MUTAGENESIS OF PHE-29; LYS-31; SER-57;
ARG-58; TYR-110 AND LYS-148, AND RNA-BINDING.;
STRAIN=ATCC 35866 / NRRL B-4488 / HD-73; PLASMID=pAW63
COMMENT [FUNCTION] Toxic component of a type III toxin-antitoxin (TA)
system (PubMed:19124776). An endoribonuclease which cleaves between
the first and second A of AAAAA sequences; it tolerates other
nucleotides in positions +2 and +4 of the consensus. Digests
cognate antitoxin RNA ToxI as shown by the 2'-3'-cyclic phosphate
at the 3' end of the 34-nt repeats and probably other RNAs
(PubMed:23267117). Inhibits growth when expressed in E.coli without
causing cell lysis; this bacteriostatic effect is neutralized by
cognate RNA antitoxin ToxI, which has 2.9 nearly identical 34
nucleotide-long repeats (PubMed:19124776). Non-cognate antitoxin
RNA from P.atrosepticum does not inhibit this toxin
(PubMed:23267117). The toxin-antitoxin pair function in plasmid
maintenance (a plasmid addiction system), but unlike its
P.atrosepticum homolog it is not seen to confer resistance to
bacteriophages (PubMed:23267117). {ECO:0000269|PubMed:19124776,
ECO:0000269|PubMed:23267117}.
[SUBUNIT] One ToxN monomer binds to a 34-nt-long single repeat of
the ToxI RNA; this complex forms a triangular heterohexameric
complex with ToxN connected by the ToxI RNA to another toxin
molecule. The ToxI repeats are cleavage products of their
precursor. The ToxI repeat forms a pseudoknot which occludes the
toxin active site. {ECO:0000269|PubMed:23267117}.
[MISCELLANEOUS] Encoded on a conjugative plasmid that includes
genes for a type IV secretion system.
{ECO:0000303|PubMed:16042811}.
[SIMILARITY] Belongs to the ToxN/AbiQ toxin family. {ECO:0000305}.
FEATURES Location/Qualifiers
source 1..194
/organism="Bacillus thuringiensis serovar kurstaki"
/plasmid=""
/serovar="kurstaki"
/db_xref="taxon:29339"
gene 1..194
/gene="toxN"
/locus_tag="pAW63_066"
Protein 1..194
/product="Endoribonuclease ToxN"
/EC_number="3.1.-.-"
/note="Toxin ToxN"
/UniProtKB_evidence="Evidence at protein level"
Region 1..194
/region_name="Mature chain"
/note="Endoribonuclease ToxN. /id=PRO_0000432893."
Region 8..166
/region_name="ToxN_toxin"
/note="Toxin ToxN, type III toxin-antitoxin system;
pfam13958"
/db_xref="CDD:433605"
Region 9..12
/region_name="Beta-strand region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Region 14..23
/region_name="Helical region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Site 29
/site_type="mutagenized"
/note="F->A: Overexpression no longer inhibits growth in
E.coli. /evidence=ECO:0000269|PubMed:23267117."
Site 31
/site_type="mutagenized"
/note="K->A: Overexpression no longer inhibits growth in
E.coli. /evidence=ECO:0000269|PubMed:23267117."
Region 38..41
/region_name="Beta-strand region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Region 51..56
/region_name="Beta-strand region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Site 57
/site_type="mutagenized"
/note="S->A: Overexpression no longer inhibits growth in
E.coli. /evidence=ECO:0000269|PubMed:23267117."
Site 58
/site_type="mutagenized"
/note="R->A: Overexpression no longer inhibits growth in
E.coli. /evidence=ECO:0000269|PubMed:23267117."
Region 65..67
/region_name="Helical region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Region 68..72
/region_name="Beta-strand region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Region 78..83
/region_name="Beta-strand region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Region 84..86
/region_name="Helical region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Region 94..97
/region_name="Beta-strand region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Region 100..106
/region_name="Helical region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Region 108..139
/region_name="Helical region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Site 110
/site_type="mutagenized"
/note="Y->A: Overexpression no longer inhibits growth in
E.coli. /evidence=ECO:0000269|PubMed:23267117."
Region 114..182
/region_name="Coiled-coil region"
/note="/evidence=ECO:0000255."
Region 147..155
/region_name="Helical region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Site 148
/site_type="mutagenized"
/note="K->A: Overexpression no longer inhibits growth in
E.coli. /evidence=ECO:0000269|PubMed:23267117."
Region 159..169
/region_name="Helical region"
/note="/evidence=ECO:0007829|PDB:4ATO."
Region 171..194
/region_name="Region of interest in the sequence"
/note="Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite."
ORIGIN
1 mtnkdnpkfh tisteyidyl readskvpfn kdeqhsrpyv gvlekinghd yfvpltsrnd
61 knfnsqvsvk lfdndekrig vllvnnmipv pekeckeidi aektaadpqy gnlmlkqylf
121 lkenmdrvtn kvekvykdvt vqgkpshkqk flkgvccdfp kleekcqeyk erdqakerdk
181 arriaymrqm grer
//