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RecName: Full=Endoribonuclease ToxN; AltName: Full=Toxin ToxN

UniProtKB/Swiss-Prot: Q3YN09.1

Identical Proteins FASTA Graphics 

LOCUS       TOXN_BACTK               194 aa            linear   BCT 27-NOV-2024
DEFINITION  RecName: Full=Endoribonuclease ToxN; AltName: Full=Toxin ToxN.
ACCESSION   Q3YN09
VERSION     Q3YN09.1
DBSOURCE    UniProtKB: locus TOXN_BACTK, accession Q3YN09;
            class: standard.
            plasmid:pAW63
            created: Apr 29, 2015.
            sequence updated: Sep 27, 2005.
            annotation updated: Nov 27, 2024.
            xrefs: DQ025752.1, AAZ06636.1, WP_000182254.1, 4ATO_A
            xrefs (non-sequence databases): PDBsum:4ATO, AlphaFoldDB:Q3YN09,
            SMR:Q3YN09, EvolutionaryTrace:Q3YN09, GO:0003723, GO:0004521,
            GO:0006276, FunFam:3.10.129.130:FF:000001, Gene3D:3.10.129.130,
            InterPro:IPR025911, InterPro:IPR053735, Pfam:PF13958
KEYWORDS    3D-structure; Coiled coil; Endonuclease; Hydrolase; Nuclease;
            Plasmid; RNA-binding; Toxin-antitoxin system.
SOURCE      Bacillus thuringiensis serovar kurstaki
  ORGANISM  Bacillus thuringiensis serovar kurstaki
            Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Bacillus; Bacillus cereus group.
REFERENCE   1  (residues 1 to 194)
  AUTHORS   Van der Auwera,G.A., Andrup,L. and Mahillon,J.
  TITLE     Conjugative plasmid pAW63 brings new insights into the genesis of
            the Bacillus anthracis virulence plasmid pXO2 and of the Bacillus
            thuringiensis plasmid pBT9727
  JOURNAL   BMC Genomics 6, 103 (2005)
   PUBMED   16042811
  REMARK    NUCLEOTIDE SEQUENCE [GENOMIC DNA].;
            STRAIN=ATCC 35866 / NRRL B-4488 / HD-73; PLASMID=pAW63
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 194)
  AUTHORS   Fineran,P.C., Blower,T.R., Foulds,I.J., Humphreys,D.P., Lilley,K.S.
            and Salmond,G.P.
  TITLE     The phage abortive infection system, ToxIN, functions as a
            protein-RNA toxin-antitoxin pair
  JOURNAL   Proc Natl Acad Sci U S A 106 (3), 894-899 (2009)
   PUBMED   19124776
  REMARK    FUNCTION, AND EXPRESSION IN E.COLI.;
            STRAIN=ATCC 35866 / NRRL B-4488 / HD-73; PLASMID=pAW63
REFERENCE   3  (residues 1 to 194)
  AUTHORS   Short,F.L., Pei,X.Y., Blower,T.R., Ong,S.L., Fineran,P.C.,
            Luisi,B.F. and Salmond,G.P.
  TITLE     Selectivity and self-assembly in the control of a bacterial toxin
            by an antitoxic noncoding RNA pseudoknot
  JOURNAL   Proc Natl Acad Sci U S A 110 (3), E241-E249 (2013)
   PUBMED   23267117
  REMARK    X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) IN COMPLEX WITH ANTITOXIN
            TOXI, FUNCTION, SUBUNIT, MUTAGENESIS OF PHE-29; LYS-31; SER-57;
            ARG-58; TYR-110 AND LYS-148, AND RNA-BINDING.;
            STRAIN=ATCC 35866 / NRRL B-4488 / HD-73; PLASMID=pAW63
COMMENT     [FUNCTION] Toxic component of a type III toxin-antitoxin (TA)
            system (PubMed:19124776). An endoribonuclease which cleaves between
            the first and second A of AAAAA sequences; it tolerates other
            nucleotides in positions +2 and +4 of the consensus. Digests
            cognate antitoxin RNA ToxI as shown by the 2'-3'-cyclic phosphate
            at the 3' end of the 34-nt repeats and probably other RNAs
            (PubMed:23267117). Inhibits growth when expressed in E.coli without
            causing cell lysis; this bacteriostatic effect is neutralized by
            cognate RNA antitoxin ToxI, which has 2.9 nearly identical 34
            nucleotide-long repeats (PubMed:19124776). Non-cognate antitoxin
            RNA from P.atrosepticum does not inhibit this toxin
            (PubMed:23267117). The toxin-antitoxin pair function in plasmid
            maintenance (a plasmid addiction system), but unlike its
            P.atrosepticum homolog it is not seen to confer resistance to
            bacteriophages (PubMed:23267117). {ECO:0000269|PubMed:19124776,
            ECO:0000269|PubMed:23267117}.
            [SUBUNIT] One ToxN monomer binds to a 34-nt-long single repeat of
            the ToxI RNA; this complex forms a triangular heterohexameric
            complex with ToxN connected by the ToxI RNA to another toxin
            molecule. The ToxI repeats are cleavage products of their
            precursor. The ToxI repeat forms a pseudoknot which occludes the
            toxin active site. {ECO:0000269|PubMed:23267117}.
            [MISCELLANEOUS] Encoded on a conjugative plasmid that includes
            genes for a type IV secretion system.
            {ECO:0000303|PubMed:16042811}.
            [SIMILARITY] Belongs to the ToxN/AbiQ toxin family. {ECO:0000305}.
FEATURES             Location/Qualifiers
     source          1..194
                     /organism="Bacillus thuringiensis serovar kurstaki"
                     /plasmid=""
                     /serovar="kurstaki"
                     /db_xref="taxon:29339"
     gene            1..194
                     /gene="toxN"
                     /locus_tag="pAW63_066"
     Protein         1..194
                     /product="Endoribonuclease ToxN"
                     /EC_number="3.1.-.-"
                     /note="Toxin ToxN"
                     /UniProtKB_evidence="Evidence at protein level"
     Region          1..194
                     /region_name="Mature chain"
                     /note="Endoribonuclease ToxN. /id=PRO_0000432893."
     Region          8..166
                     /region_name="ToxN_toxin"
                     /note="Toxin ToxN, type III toxin-antitoxin system;
                     pfam13958"
                     /db_xref="CDD:433605"
     Region          9..12
                     /region_name="Beta-strand region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Region          14..23
                     /region_name="Helical region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Site            29
                     /site_type="mutagenized"
                     /note="F->A: Overexpression no longer inhibits growth in
                     E.coli. /evidence=ECO:0000269|PubMed:23267117."
     Site            31
                     /site_type="mutagenized"
                     /note="K->A: Overexpression no longer inhibits growth in
                     E.coli. /evidence=ECO:0000269|PubMed:23267117."
     Region          38..41
                     /region_name="Beta-strand region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Region          51..56
                     /region_name="Beta-strand region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Site            57
                     /site_type="mutagenized"
                     /note="S->A: Overexpression no longer inhibits growth in
                     E.coli. /evidence=ECO:0000269|PubMed:23267117."
     Site            58
                     /site_type="mutagenized"
                     /note="R->A: Overexpression no longer inhibits growth in
                     E.coli. /evidence=ECO:0000269|PubMed:23267117."
     Region          65..67
                     /region_name="Helical region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Region          68..72
                     /region_name="Beta-strand region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Region          78..83
                     /region_name="Beta-strand region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Region          84..86
                     /region_name="Helical region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Region          94..97
                     /region_name="Beta-strand region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Region          100..106
                     /region_name="Helical region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Region          108..139
                     /region_name="Helical region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Site            110
                     /site_type="mutagenized"
                     /note="Y->A: Overexpression no longer inhibits growth in
                     E.coli. /evidence=ECO:0000269|PubMed:23267117."
     Region          114..182
                     /region_name="Coiled-coil region"
                     /note="/evidence=ECO:0000255."
     Region          147..155
                     /region_name="Helical region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Site            148
                     /site_type="mutagenized"
                     /note="K->A: Overexpression no longer inhibits growth in
                     E.coli. /evidence=ECO:0000269|PubMed:23267117."
     Region          159..169
                     /region_name="Helical region"
                     /note="/evidence=ECO:0007829|PDB:4ATO."
     Region          171..194
                     /region_name="Region of interest in the sequence"
                     /note="Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite."
ORIGIN      
        1 mtnkdnpkfh tisteyidyl readskvpfn kdeqhsrpyv gvlekinghd yfvpltsrnd
       61 knfnsqvsvk lfdndekrig vllvnnmipv pekeckeidi aektaadpqy gnlmlkqylf
      121 lkenmdrvtn kvekvykdvt vqgkpshkqk flkgvccdfp kleekcqeyk erdqakerdk
      181 arriaymrqm grer
//
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