Protein Family Models

This family of domains contain a P-loop motif that is characteristic of the AAA superfamily. Many of the proteins in this family are conjugative transfer proteins. There is a Walker A and Walker B. (from Pfam)

Date:

2024-08-14

Date:

2024-08-14

Relaxases are DNA strand transferases which function during the conjugative cell to cell DNA transfer. TrwC binds to the origin of transfer (oriT) and melts the double helix. [1]. 16540117. Unveiling the Molecular Mechanism of a Conjugative Relaxase: The Structure of TrwC Complexed with a 27-mer DNA Comprising the Recognition Hairpin and the Cleavage Site. Boer R, Russi S, Guasch A, Lucas M, Blanco AG, Perez-Luque R, Coll M, de la Cruz F;. J Mol Biol. 2006;358:857-869. [2]. 14625590. Recognition and processing of the origin of transfer DNA by conjugative relaxase TrwC. Guasch A, Lucas M, Moncalian G, Cabezas M, Perez-Luque R, Gomis-Ruth FX, de la Cruz F, Coll M;. Nat Struct Biol. 2003;10:1002-1010. [3]. 8950267. Functional domains in protein TrwC of plasmid R388: dissected DNA strand transferase and DNA helicase activities reconstitute protein function. Llosa M, Grandoso G, Hernando MA, de la Cruz F;. J Mol Biol. 1996;264:56-67. (from Pfam)

Date:

2024-10-16

Helicase activity for this family has been demonstrated [1] and NTPase activity [2]. This helicase has multiple roles at different stages of viral RNA replication, as dissected by mutational analysis [3]. This family is discussed pages 388-391. [1]. 8269709. Evolution and taxonomy of positive-strand RNA viruses: implications of comparative analysis of amino acid sequences. Koonin EV, Dolja VV;. Crit Rev Biochem Mol Biol 1993;28:375-430. [2]. 10217401. RNA helicase activity of Semliki Forest virus replicase protein NSP2. Gomez de Cedron M, Ehsani N, Mikkola ML, Garcia JA, Kaariainen L;. FEBS Lett 1999;448:19-22. [3]. 8057461. ATPase and GTPase activities associated with Semliki Forest virus nonstructural protein nsP2. Rikkonen M, Peranen J, Kaariainen L;. J Virol 1994;68:5804-5810. [4]. 10982322. Helicase and capping enzyme active site mutations in brome mosaic virus protein 1a cause defects in template recruitment, negative-strand RNA synthesis, and viral RNA capping. Ahola T, den Boon JA, Ahlquist P;. J Virol 2000;74:8803-8811. (from Pfam)

Date:

2024-10-16

Gene:

mobF

Date:

2023-01-23