Protein Family Models

This entry represents a domain found in N-terminal in MFE-2 hydratase 2 and related enzymes. This domain is housing the cavity for the aliphatic acyl part of the substrate molecule. The flexibility of region within this domain plays a role in substrate binding in mammalian hydratase 2 [2]. Paper describing PDB structure 1pn2. [1]. 15051722. A two-domain structure of one subunit explains unique features of eukaryotic hydratase 2. Koski MK, Haapalainen AM, Hiltunen JK, Glumoff T;. J Biol Chem. 2004;279:24666-24672. Paper describing PDB structure 1s9c. [2]. 15644212. Crystal structure of 2-enoyl-CoA hydratase 2 from human peroxisomal multifunctional enzyme type 2. Koski KM, Haapalainen AM, Hiltunen JK, Glumoff T;. J Mol Biol. 2005;345:1157-1169. Paper describing PDB structure 2c2i. [3]. 16963641. Structure and function of Rv0130, a conserved hypothetical protein from Mycobacterium tuberculosis. Johansson P, Castell A, Jones TA, Backbro K;. Protein Sci. 2006;15:2300-2309. Paper describing PDB structure 2cdh. [4]. 16513976. Architecture of a fungal fatty acid synthase at 5 A resolution. Jenni S, Leibundgut M, Maier T, Ban N;. Science. 2006;311:1263-1267. Paper describing PDB structure 2uv8. [5]. 17431182. Structural basis for substrate delivery by acyl carrier protein in the yeast fatty acid synthase. Leibundgut M, Jenni S, Frick C, Ban N;. Science. 2007;316:288-290. (from Pfam)

Date:

2024-10-16

This domain is found in Enoyl-(Acyl carrier protein) reductases. (from Pfam)

Date:

2024-08-14

This enzymatic domain is part of bacterial polyketide synthases and catalyses the first step in the reductive modification of the beta-carbonyl centres in the growing polyketide chain. It uses NADPH to reduce the keto group to a hydroxy group [1]. [1]. 23790488. Structural and stereochemical analysis of a modular polyketide synthase ketoreductase domain required for the generation of a cis-alkene. Bonnett SA, Whicher JR, Papireddy K, Florova G, Smith JL, Reynolds KA;. Chem Biol. 2013;20:772-783. (from Pfam)

Date:

2024-10-16

This domain is involved in binding sterols. It is found in the SCP2 protein Swiss:P22307, as well as the C terminus of Swiss:P51659 the enzyme estradiol 17 beta-dehydrogenase EC:1.1.1.62. The UNC-24 protein Swiss:Q17372 contains an SPFH domain Pfam:PF01145 [2]. [1]. 8243660. NMR determination of the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2. Szyperski T, Scheek S, Johansson J, Assmann G, Seedorf U, Wuthrich K;. FEBS Lett 1993;335:18-26. [2]. 8667025. The Caenorhabditis elegans behavioral gene unc-24 encodes a novel bipartite protein similar to both erythrocyte band 7.2 (stomatin) and nonspecific lipid transfer protein. Barnes TM, Jin Y, Horvitz HR, Ruvkun G, Hekimi S;. J Neurochem 1996;67:46-57. (from Pfam)

Date:

2024-10-16

The maoC gene is part of a operon with maoA which is involved in the synthesis of monoamine oxidase [1]. The MaoC protein is found to share similarity with a wide variety of enzymes; estradiol 17 beta-dehydrogenase 4, peroxisomal hydratase-dehydrogenase-epimerase, fatty acid synthase beta subunit. Several bacterial proteins that are composed solely of this domain have (R)-specific enoyl-CoA hydratase activity [2]. This domain is also present in the NodN nodulation protein N. [1]. 8647101. A monoamine-regulated Klebsiella aerogenes operon containing the monoamine oxidase structural gene (maoA) and the maoC gene. Sugino H, Sasaki M, Azakami H, Yamashita M, Murooka Y. J Bacteriol 1992;174:2485-2492. [2]. 9457873. Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae. Fukui T, Shiomi N, Doi Y;. J Bacteriol. 1998;180:667-673. (from Pfam)

Date:

2024-10-16

This family contains a wide variety of dehydrogenases. [1]. 9735295. The refined crystal structure of Drosophila lebanonensis alcohol dehydrogenase at 1.9 A resolution. Benach J, Atrian S, Gonzalez-Duarte R, Ladenstein R;. J Mol Biol 1998;282:383-399. [2]. 10387002. Structure of tropinone reductase-II complexed with NADP+ and pseudotropine at 1.9 A resolution: implication for stereospecific substrate binding and catalysis. Yamashita A, Kato H, Wakatsuki S, Tomizaki T, Nakatsu T, Nakajima K, Hashimoto T, Yamada Y, Oda J;. Biochemistry 1999;38:7630-7637. (from Pfam)

Date:

2024-10-16

peroxisomal multifunctional enzyme type 2 (MFE-2) is a bifunctional enzyme that catalyzes the formation of 3-ketoacyl-CoA intermediates from straight-chain, 2-methyl-branched-chain fatty acids bile acid intermediates

Date:

2023-09-17