Protein Family Models

This protein is an initiator of plasmid replication [1-6]. RepB possesses nicking-closing (topoisomerase I) like activity. It is also able to perform a strand transfer reaction on ssDNA that contains its target. This entry also includes RepA which is an E.coli protein involved in plasmid replication. The RepA protein binds to DNA repeats that flank the repA gene [3,4]. These proteins consist of two domains containing a winged helix-turn-helix motif, the N-terminal one, WH1, represented in this entry, is involved in the dimerization of RepE and an auxiliary binding to DNA. The C-terminal one, WH2, is related to a sequence-specific binding to the common 8 bp of the iteron [6]. [1]. 9618448. Replication and control of circular bacterial plasmids. del Solar G, Giraldo R, Ruiz-Echevarria MJ, Espinosa M, Diaz-Orejas R;. Microbiol Mol Biol Rev 1998;62:434-464. [2]. 9180376. Initiation of replication of plasmid pMV158: mechanisms of DNA strand-transfer reactions mediated by the initiator RepB protein. Moscoso M, Eritja R, Espinosa M;. J Mol Biol 1997;268:840-856. [3]. 8320218. Regulatory interactions between RepA, an essential replication protein, and the DNA repeats of RepFIB from plasmid P307. Spiers AJ, Bhana N, Bergquist PL;. J Bacteriol 1993;175:4016-4024. [4]. 3949778. P1 plasmid replication. Purification and DNA-binding activity of the replication protein RepA. Abeles AL;. J Biol Chem 1986;261:3548-3555. [5]. 15336485. Twenty years of the pPS10 replicon: insights on the molecular mechanism for the activation of DNA replication in iteron-containing bacterial plasmids. Giraldo R, Fernandez-Tresguerres ME;. Plasmid. 2004;52:69. TRUNCATED at 1650 bytes (from Pfam)

Date:

2024-10-16

replication initiation protein is essential for plasmid DNA replication

Date:

2022-11-03