Protein Family Models

This domain occurs at the C-terminus of transposases including E. coli tnpA (Swiss:Q46731). TnpA encodes a transposase and an inhibitor protein, the inhibitor only differs from the transposase by the absence of the N-terminal 55 amino acids, which includes most of this domain [1]. This domain consists of alpha helices and turns, and functions as a DNA-binding domain [2]. [1]. 10207011. The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9-A resolution. Davies DR, Braam LM, Reznikoff WS, Rayment I;. J Biol Chem 1999;274:11904-11913. [2]. 15102449. Structure/function insights into Tn5 transposition. Steiniger-White M, Rayment I, Reznikoff WS;. Curr Opin Struct Biol. 2004;14:50-57. (from Pfam)

Date:

2024-10-16

Tn5 belongs to the IS4 family of mobile element. This HMM describes a dimerization domain found at the C-terminus of IS4 family transposases.

Date:

2024-10-16

Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction [3]. This family contains transposases for IS4 Swiss:P03835 [1], IS421 Swiss:P11901 [2], IS5377 Swiss:Q45620, IS427 [4], IS402 [5], IS1355 Swiss:O69604, IS5, which was original isolated in bacteriophage lambda [6]. [1]. 6268937. The sequence of IS4. Klaer R, Kuhn S, Tillmann E, Fritz HJ, Starlinger P;. Mol Gen Genet 1981;181:169-175. [2]. 2542093. IS421, a new insertion sequence in Escherichia coli. Sato S, Nakada Y, Shiratsuchi A;. FEBS Lett 1989;249:21-26. [3]. 8335077. Identification and analysis of the gas vesicle gene cluster on an unstable plasmid of Halobacterium halobium. DasSarma S;. Plasmid 1993;29:1-9. [4]. 10207011. The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9-A resolution. Davies DR, Braam LM, Reznikoff WS, Rayment I;. J Biol Chem 1999;274:11904-11913. [5]. 1963949. Nucleotide sequence analysis of IS427 and its target sites in Agrobacterium tumefaciens T37. De Meirsman C, Van Soom C, Verreth C, Van Gool A, Vanderleyden J;. Plasmid 1990;24:227-234. [6]. 1650732. Nucleotide sequence of IS402 from Pseudomonas cepacia. Ferrante AA, Lessie TG;. Gene 1991;102:143-144. [7]. 6281654. Structural analysis of insertion sequence IS5. Kroger M, Hobom G;. Nature 1982;297:159-162. (from Pfam)

Date:

2024-10-16

transposase binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Date:

2020-12-14

Date:

2021-03-03

Date:

2019-09-10