Protein Family Models

Replication proteins (rep) are involved in plasmid replication. The Rep protein binds to the plasmid DNA and nicks it at the double strand origin (dso) of replication. The 3'-hydroxyl end created is extended by the host DNA replicase, and the 5' end is displaced during synthesis. At the end of one replication round, Rep introduces a second single stranded break at the dso and ligates the ssDNA extremities generating one double-stranded plasmid and one circular ssDNA form. Complementary strand synthesis of the circular ssDNA is usually initiated at the single-stranded origin by the host RNA polymerase [1]. [1]. 9570403. A rolling circle replication initiator protein with a. nucleotidyl-transferase activity encoded by the plasmid pGT5. from the hyperthermophilic archaeon Pyrococcus abyssi.. Marsin S, Forterre P;. Mol Microbiol 1998;27:1183-1192. (from Pfam)

Date:

2024-08-14

protein rep is a replication initiation protein that binds to the origin of replication and produces a nick in a specific site of the plasmid, resulting in single-strand replication by a rolling circle mechanism

Date:

2018-10-11