Protein Family Models

RctB is a highly conserved 75.3 kD protein (658 residues), which is unique to the Vibrionaceae. The first 500 amino acids of RctB are sufficient to mediate oriCII-based replication and its C-terminal 165 residues may mediate regulatory processes. RctB contains at least three DNA binding winged-helix-turn-helix motifs, and mutations within any of these severely compromise biological activity. This entry describes domain 1 located at the N-terminal region of RctB proteins. Mutational analysis show that it binds oriCII DNA, and that this function is critical for the capacity of RctB to mediate oriCII-based replication [1]. [1]. 28031373. The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators. Orlova N, Gerding M, Ivashkiv O, Olinares PDB, Chait BT, Waldor MK, Jeruzalmi D;. Nucleic Acids Res. 2017;45:3724-3737. (from Pfam)

Date:

2024-10-16

This region is found in the replication initiator protein RctB from Vibrio cholerae (Swiss:Q9KNG2) and similar sequences predominantly found in Vibrionales. RctB mediates oriCII-based replication and other regulatory processes. This protein is organised into four domains and adopts a dimeric configuration. This entry represents the two central domains, one consisting of a seven-stranded beta- sheet that forms the interface of the dimer by forming a swapped configuration with the same domain of the other monomer. It seems to be involved in DNA binding [1]. [1]. 28031373. The replication initiator of the cholera pathogen's second chromosome shows structural similarity to plasmid initiators. Orlova N, Gerding M, Ivashkiv O, Olinares PDB, Chait BT, Waldor MK, Jeruzalmi D;. Nucleic Acids Res. 2017;45:3724-3737. (from Pfam)

Date:

2024-10-16