Protein Family Models

RNA polymerases catalyse the DNA dependent polymerisation of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain, domain 3, represents the pore domain. The 3' end of RNA is positioned close to this domain. The pore delimited by this domain is thought to act as a channel through which nucleotides enter the active site and/or where the 3' end of the RNA may be extruded during back-tracking [1,2]. [1]. 8910400. Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase. Severinov K, Mustaev A, Kukarin A, Muzzin O, Bass I, Darst SA, Goldfarb A;. J Biol Chem 1996;271:27969-27974. [2]. 11313498. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Cramer P, Bushnell DA, Kornberg RD;. Science 2001;292:1863-1876. (from Pfam)

Date:

2024-10-16

RNA polymerases catalyse the DNA dependent polymerisation of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain, domain 4, represents the funnel domain. The funnel contain the binding site for some elongation factors [1,2]. [1]. 8910400. Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase. Severinov K, Mustaev A, Kukarin A, Muzzin O, Bass I, Darst SA, Goldfarb A;. J Biol Chem 1996;271:27969-27974. [2]. 11313498. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Cramer P, Bushnell DA, Kornberg RD;. Science 2001;292:1863-1876. (from Pfam)

Date:

2024-10-16

RNA polymerases catalyse the DNA dependent polymerisation of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain, domain 5, represents the discontinuous cleft domain that is required to from the central cleft or channel where the DNA is bound [1,2]. [1]. 8910400. Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase. Severinov K, Mustaev A, Kukarin A, Muzzin O, Bass I, Darst SA, Goldfarb A;. J Biol Chem 1996;271:27969-27974. [2]. 11313498. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Cramer P, Bushnell DA, Kornberg RD;. Science 2001;292:1863-1876. (from Pfam)

Date:

2024-10-16

DNA-directed RNA polymerase subunit beta' catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Date:

2019-03-13

The family consists of the product of the rpoC2 gene, a subunit of DNA-directed RNA polymerase of cyanobacteria and chloroplasts. RpoC2 corresponds largely to the C-terminal region of the RpoC (the beta' subunit) of other bacteria. Members of this family are designated beta'' in chloroplasts/plastids, and beta' (confusingly) in Cyanobacteria, where RpoC1 is called beta' in chloroplasts/plastids and gamma in Cyanobacteria. We prefer to name this family beta'', after its organellar members, to emphasize that this RpoC1 and RpoC2 together replace RpoC in other bacteria.

Date:

2024-05-30

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Date:

2020-10-26