Protein Family Models

This domain is found in centromere-binding protein (SopB). SopB displays an intriguing range of DNA-binding properties essential for partition; it binds the centromere to form a partition complex, which recruits NTPase (SopA), and it also inhibits SopA polymerization. The domain has a helix-turn-helix (HTH) structure and is thought to be the specific DNA-binding domain mainly through residues from the recognition helix, alpha 3, of the HTH. The domain has show structural similarity to the DNA-binding domains of P1 ParB and KorB [1]. [1]. 20236989. Insight into F plasmid DNA segregation revealed by structures of SopB and SopB-DNA complexes. Schumacher MA, Piro KM, Xu W;. Nucleic Acids Res. 2010;38:4514-4526. (from Pfam)

Date:

2024-10-16

Proteins containing this domain include Escherichia coli plasmid protein ParB and mammalian Sulfiredoxin-1. ParB is involved in chromosome partition. It localises to both poles of the predivisional cell following completion of DNA replication [1]. Sulfiredoxin-1 contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in the peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4 [2]. [1]. 12603730. Productive interaction between the chromosome partitioning proteins, ParA and ParB, is required for the progression of the cell cycle in Caulobacter crescentus. Figge RM, Easter J, Gober JW;. Mol Microbiol. 2003;47:1225-1237. [2]. 15448164. Characterization of mammalian sulfiredoxin and its reactivation of hyperoxidized peroxiredoxin through reduction of cysteine sulfinic acid in the active site to cysteine. Chang TS, Jeong W, Woo HA, Lee SM, Park S, Rhee SG;. J Biol Chem. 2004;279:50994-51001. (from Pfam)

Date:

2024-10-16

PRK13698 family protein

Date:

2017-03-02

Date:

2020-10-26

This HMM represents the most well-conserved core of a set of chromosomal and plasmid partition proteins related to ParB, including Spo0J, RepB, and SopB. Spo0J has been shown to bind a specific DNA sequence that, when introduced into a plasmid, can serve as partition site. Study of RepB, which has nicking-closing activity, suggests that it forms a transient protein-DNA covalent intermediate during the strand transfer reaction.

Date:

2021-04-27