Protein Family Models

These lipopolysaccharide kinases are related to protein kinases Pfam:PF00069. This family includes waaP (rfaP) gene product is required for the addition of phosphate to O-4 of the first heptose residue of the lipopolysaccharide (LPS) inner core region. It has previously been shown that WaaP is necessary for resistance to hydrophobic and polycationic antimicrobials in E. coli and that it is required for virulence in invasive strains of S. enterica [1]. [1]. 11069912. Purification and characterization of WaaP from Escherichia coli, a lipopolysaccharide kinase essential for outer membrane stability. Yethon JA, Whitfield C;. J Biol Chem 2001;276:5498-5504. [2]. 12021457. Lipopolysaccharide phosphorylating enzymes encoded in the genomes of Gram-negative bacteria are related to the eukaryotic protein kinases. Krupa A, Srinivasan N;. Protein Sci 2002;11:1580-1584. [3]. 10531340. A Haemophilus influenzae gene that encodes a membrane bound 3-deoxy-D-manno-octulosonic acid (Kdo) kinase. Possible involvement of kdo phosphorylation in bacterial virulence. White KA, Lin S, Cotter RJ, Raetz CR;. J Biol Chem 1999;274:31391-31400. (from Pfam)

Date:

2024-10-16

This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation [2]. This family also includes homoserine kinase. This family is related to fructosamine kinase Pfam:PF03881. [1]. 2163618. Cloning of aminoglycoside phosphotransferase (APH) gene from antibiotic- producing strain of Bacillus circulans into a high-expression vector, pKK223-3. Purification, properties and location of the enzyme [published erratum appears in Biochem J 1991 Feb 1. Sarwar M, Akhtar M;. Biochem J 1990;268:671-677. [2]. 2167474. PCR cloning of a streptomycin phosphotransferase (aphE) gene from Streptomyces griseus ATCC 12475. Trower MK, Clark KG;. Nucleic Acids Res 1990;18:4615-4615. (from Pfam)

Date:

2024-10-16

This is a family of atypical serine kinases which are found in archaea, bacteria and eukaryotes. Activity of Rio1 is vital in Saccharomyces cerevisiae for the processing of ribosomal RNA, as well as for proper cell cycle progression and chromosome maintenance. The structure of RIO1 has been determined [4]. [1]. 12021457. Lipopolysaccharide phosphorylating enzymes encoded in the genomes of Gram-negative bacteria are related to the eukaryotic protein kinases. Krupa A, Srinivasan N;. Protein Sci 2002;11:1580-1584. [2]. 12612080. Late cytoplasmic maturation of the small ribosomal subunit requires RIO proteins in Saccharomyces cerevisiae. Vanrobays E, Gelugne JP, Gleizes PE, Caizergues-Ferrer M;. Mol Cell Biol 2003;23:2083-2095. [3]. 12690111. Rio2p: An evolutionarily conserved, low-abundant protein kinase essential for processing of 20S Pre-rRNA in Saccharomyces cerevisiae. Geerlings TH, Faber AW, Bister M, Vos JC, Raue HA;. 0;0:0-0. [4]. 16008568. Structure and activity of the atypical serine kinase Rio1. Laronde-Leblanc N, Guszczynski T, Copeland T, Wlodawer A;. FEBS J 2005;272:3698-3713. (from Pfam)

Date:

2024-10-16

Catalyzes the phosphorylation of 3-deoxy-D-manno-octulosonic acid

Date:

2021-09-01