F420-0:Gamma-glutamyl ligase (EC:6.3.2.-) is an enzyme involved in F420 biosynthesis pathway. It catalyses the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0). This reaction produces polyglutamated F420 derivatives. GTP + F420-0 + n L-glutamate -> GDP + phosphate + F420-n [1]. 15215601. Identification and cloning of the gene involved in the final step of chlortetracycline biosynthesis in Streptomyces aureofaciens. Nakano T, Miyake K, Endo H, Dairi T, Mizukami T, Katsumata R;. Biosci Biotechnol Biochem. 2004;68:1345-1352. [2]. 8577249. Molecular characterization of the lincomycin-production gene cluster of Streptomyces lincolnensis 78-11. Peschke U, Schmidt H, Zhang HZ, Piepersberg W;. Mol Microbiol. 1995;16:1137-1156. [3]. 12867481. Methanococcus jannaschii coenzyme F420 analogs contain a terminal alpha-linked glutamate. Graupner M, White RH;. J Bacteriol. 2003;185:4662-4665. [4]. 2110564. DNA photoreactivating enzyme from the cyanobacterium Anacystis nidulans. Eker AP, Kooiman P, Hessels JK, Yasui A;. J Biol Chem. 1990;265:8009-8015. (from Pfam)
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- 2024-10-16