Bacterial periplasmic or secreted endonuclease I (EC:3.1.21.1) E. coli endonuclease I (EndoI) is a sequence independent endonuclease located in the periplasm. It is inhibited by different RNA species. It is thought to normally generate double strand breaks in DNA, except in the presence of high salt concentrations and RNA, when it generates single strand breaks in DNA. Its biological role is unknown [1]. Other family members are known to be extracellular [2]. This family also includes a non-specific, Mg2+ activated ribonuclease precursor (Swiss:Q03091) [3]. [1]. 7867949. The periplasmic endonuclease I of Escherichia coli has amino-acid sequence homology to the extracellular DNases of Vibrio cholerae and Aeromonas hydrophila. Jekel M, Wackernagel W;. Gene 1995;154:55-59. [2]. 3036665. Extracellular proteins of Vibrio cholerae: molecular cloning, nucleotide sequence and characterization of the deoxyribonuclease (DNase) together with its periplasmic localization in Escherichia coli K-12. Focareta T, Manning PA;. Gene 1987;53:31-40. [3]. 1396690. Gene cloning and characterization of a novel extracellular ribonuclease of Bacillus subtilis. Nakamura A, Koide Y, Miyazaki H, Kitamura A, Masaki H, Beppu T, Uozumi T;. Eur J Biochem 1992;209:121-127. (from Pfam)
GO Terms:- Molecular Function:
- nuclease activity (GO:0004518)
- Date:
- 2024-10-16