Protein Family Models

The broad host-range plasmid RK2 is able to replicate in and be inherited in a stable manner in diverse Gram-negative bacterial species. It encodes a number of co-ordinately regulated operons including a central control korF1 operon that represses the kfrA operon. The KfrA polypeptide is a site-specific DNA-binding protein whose operator overlaps the kfrA promoter. The N-terminus, containing an helix-turn-helix motif, is essential for function. Downstream from this family is an extended coiled-coil domain containing a heptad repeat segment which is probably responsible for formation of multimers, and may provide an example of a bridge to host structures required for plasmid partitioning [2]. [1]. 9245806. Divergence and conservation of the partitioning and global regulation functions in the central control region of the IncP plasmids RK2 and R751. Macartney DP, Williams DR, Stafford T, Thomas CM;. Microbiology. 1997;143:2167-2177. [2]. 1602476. kfrA gene of broad host range plasmid RK2 encodes a novel DNA-binding protein. Jagura-Burdzy G, Thomas CM;. J Mol Biol. 1992;225:651-660. (from Pfam)

Date:

2024-10-16

Cenp-F, a centromeric kinetochore, microtubule-binding protein consisting of two 1,600-amino acid-long coils, is essential for the full functioning of the mitotic checkpoint pathway [1,2]. There are several leucine-rich repeats along the sequence of LEK1 that are considered to be zippers, though they do not appear to be binding DNA directly in this instance [2]. [1]. 7542657. CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis. Liao H, Winkfein RJ, Mack G, Rattner JB, Yen TJ;. J Cell Biol. 1995;130:507-518. [2]. 14555653. LEK1 is a potential inhibitor of pocket protein-mediated cellular processes. Ashe M, Pabon-Pena L, Dees E, Price KL, Bader D;. J Biol Chem. 2004;279:664-676. (from Pfam)

Date:

2024-10-16

DNA-binding protein similar to plasmid KfrA proteins

Date:

2021-02-05