Protein Family Models

This family (also known as the ABI (abortive infection) family) contains putative IMPs and has homologues in all three domains of life, including Rce1 from S. cerevisiae [1]. Rce1 is a type II CAAX prenyl protease that processes all farnesylated and geranylgeranylated CAAX proteins. It is an integral membrane endoprotease localized to the endoplasmic reticulum that mediates the cleavage of the carboxyl-terminal three amino acids from CaaX proteins. It is involved in processing the Ras family of small GTPases, the gamma-subunit of heterotrimeric GTPases, nuclear lamins, and protein kinases and phosphatases [2]. Three residues of S. cerevisiae Rce1 -E156, H194 and H248- are critical for catalysis [3]. The structure of Rce1 from the archaea Methanococcus (MmRce1) suggests that this group of proteins represents a novel IMP (intramembrane protease) family, the glutamate IMPs [4]. There is a conserved sequence motif EExxxR. [1]. 20154137. The abi proteins and their involvement in bacteriocin. self-immunity.. Kjos M, Snipen L, Salehian Z, Nes IF, Diep DB;. J Bacteriol. 2010;192:2068-2076.. [2]. 29424242. Rce1: mechanism and inhibition.. Hampton SE, Dore TM, Schmidt WK;. Crit Rev Biochem Mol Biol. 2018;53:157-174.. [3]. 16361710. Mutational analysis of the ras converting enzyme reveals a. requirement for glutamate and histidine residues.. Plummer LJ, Hildebrandt ER, Porter SB, Rogers VA, McCracken J,. Schmidt WK;. J Biol Chem. 2006;281:4596-4605.. [4]. 24291792. Mechanism of farnesylated CAAX protein processing by the. intramembrane protease Rce1.. Manolaridis I, Kulkarni K, Dodd RB, Ogasawara S, Zhang Z, Bineva. G, Reilly NO, Han. TRUNCATED at 1650 bytes (from Pfam)

Date:

2024-08-14

Members of this family are MroQ, an intramembrane glutamic protease related to the myxosortases and to the eukaryotic type II CAAX prenyl protease Rce1. MroQ often is encoded within 6 kilobases of the agr (accessory gene regulator) quorum-sensing system, and it is involved in leader peptide removal from the autoinducer peptide (AIP) after it is cleaved and cyclized at a Cys side chain the cysteine peptidase AgrB.

Gene:

mroQ

Date:

2024-06-11

MroQ, encoded in Staphylococcus aureus near genes of the abr quorum-sensing sytem, appears to participate in its regulation of various virulence-related functions. By homology, MroQ appears to be a glutamic endopeptidase with an intramembrane active site, but what MroQ cleaves remains unknown.

Gene:

mroQ

Date:

2023-07-14

CPBP (CAAX proteases and bacteriocin-processing enzymes) family intramembrane protease similar to Saccharomyces cerevisiae Rce1, a type II CAAX prenyl protease that processes all farnesylated and geranylgeranylated CAAX proteins. It is an integral membrane endoprotease that belongs to the glutamate IMPs, sharing a conserved sequence motif EExxxR

Date:

2024-04-25