Protein Family Models

5-formyltetrahydrofolate cyclo-ligase or methenyl-THF synthetase EC:6.3.3.2 catalyses the interchange of 5-formyltetrahydrofolate (5-FTHF) to 5-10-methenyltetrahydrofolate, this requires ATP and Mg2+ [1]. 5-FTHF is used in chemotherapy where it is clinically known as Leucovorin [2]. [1]. 8522195. Cloning and characterization of the human 5,10-methenyltetrahydrofolate synthetase-encoding cDNA. Dayan A, Bertrand R, Beauchemin M, Chahla D, Mamo A, Filion M, Skup D, Massie B, Jolivet J;. Gene 1995;165:307-311. [2]. 8034591. Primary structure and tetrahydropteroylglutamate binding site of rabbit liver cytosolic 5,10-methenyltetrahydrofolate synthetase. Maras B, Stover P, Valiante S, Barra D, Schirch V;. J Biol Chem 1994;269:18429-18433. (from Pfam)

Date:

2024-10-16

This enzyme, 5,10-methenyltetrahydrofolate synthetase, is also called 5-formyltetrahydrofolate cycloligase. Function of bacterial proteins in this family was inferred originally from the known activity of eukaryotic homologs. Recently, activity was shown explicitly for the member from Mycoplasma pneumonia. Members of this family from alpha- and gamma-proteobacteria, designated ygfA, are often found in an operon with 6S structural RNA, and show a similar pattern of high expression during stationary phase. The function may be to deplete folate to slow 1-carbon biosynthetic metabolism.

Date:

2021-04-27

5-formyltetrahydrofolate cyclo-ligase catalyzes the irreversible conversion of 5-formyltetrahydrofolate (5-FTHF) to 5,10-methenyltetrahydrofolate, part of the folate metabolism

Date:

2022-09-06