Protein Family Models

PFOR_II is a core domain of the anaerobic enzyme pyruvate:ferredoxin oxidoreductase and is necessary for inter subunit contacts in conjunction with domains I and IV [1,2]. [1]. 10048931. Crystal structures of the key anaerobic enzyme pyruvate:ferredoxin oxidoreductase, free and in complex with pyruvate. Chabriere E, Charon MH, Volbeda A, Pieulle L, Hatchikian EC, Fontecilla-Camps JC;. Nat Struct Biol 1999;6:182-190. [2]. 20956531. Identification and characterization of oxalate oxidoreductase, a novel thiamine pyrophosphate-dependent 2-oxoacid oxidoreductase that enables anaerobic growth on oxalate. Pierce E, Becker DF, Ragsdale SW;. J Biol Chem. 2010;285:40515-40524. (from Pfam)

Date:

2024-10-16

The C-terminal domain of transketolase has been proposed as a regulatory molecule binding site [2]. [1]. 8176731. Refined structure of transketolase from Saccharomyces cerevisiae at 2.0 A resolution. Nikkola M, Lindqvist Y, Schneider G;. J Mol Biol 1994;238:387-404. [2]. 1628611. Three-dimensional structure of transketolase, a thiamine diphosphate dependent enzyme, at 2.5 A resolution. Lindqvist Y, Schneider G, Ermler U, Sundstrom M;. EMBO J 1992;11:2373-2379. (from Pfam)

Date:

2024-10-16

This family includes the N terminal structural domain of the pyruvate ferredoxin oxidoreductase. This domain binds thiamine diphosphate, and along with domains II and IV, is involved in inter subunit contacts [3]. The family also includes pyruvate flavodoxin oxidoreductase as encoded by the nifJ gene in cyanobacterium which is required for growth on molecular nitrogen when iron is limited [2]. [1]. 8550425. Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima. Kletzin A, Adams MW;. J Bacteriol 1996;178:248-257. [2]. 8415612. Growth of the cyanobacterium Anabaena on molecular nitrogen: NifJ is required when iron is limited. Bauer CC, Scappino L, Haselkorn R;. Proc Natl Acad Sci U S A 1993;90:8812-8816. [3]. 10048931. Crystal structures of the key anaerobic enzyme pyruvate:ferredoxin oxidoreductase, free and in complex with pyruvate. Chabriere E, Charon MH, Volbeda A, Pieulle L, Hatchikian EC, Fontecilla-Camps JC;. Nat Struct Biol 1999;6:182-190. (from Pfam)

Date:

2024-10-16

This family includes a region of the large protein pyruvate-flavodoxin oxidoreductase and the whole pyruvate ferredoxin oxidoreductase gamma subunit protein. It is not known whether the gamma subunit has a catalytic or regulatory role. Pyruvate oxidoreductase (POR) catalyses the final step in the fermentation of carbohydrates in anaerobic microorganisms [1]. This involves the oxidative decarboxylation of pyruvate with the participation of thiamine followed by the transfer of an acetyl moiety to coenzyme A for the synthesis of acetyl-CoA [1]. The family also includes pyruvate flavodoxin oxidoreductase as encoded by the nifJ gene in cyanobacterium which is required for growth on molecular nitrogen when iron is limited [2]. [1]. 8550425. Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima. Kletzin A, Adams MW;. J Bacteriol 1996;178:248-257. [2]. 8415612. Growth of the cyanobacterium Anabaena on molecular nitrogen: NifJ is required when iron is limited. Bauer CC, Scappino L, Haselkorn R;. Proc Natl Acad Sci U S A 1993;90:8812-8816. (from Pfam)

Date:

2024-10-16

2-oxoacid:acceptor oxidoreductase subunit alpha such as Mycobacterium tuberculosis 2-oxoglutarate oxidoreductase subunit KorA, which is a component of the enzyme that catalyzes the CoA-dependent oxidative decarboxylation of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA

Date:

2018-01-08

This family of proteins contains a C-terminal thiamine diphosphate (TPP) binding domain typical of flavodoxin/ferredoxin oxidoreductases (PF01855) as well as an N-terminal domain similar to the gamma subunit of the same group of oxidoreductases (PF01558). The genes represented by this model are always found in association with a neighboring gene for a beta subunit (TIGR02177) which also occurs in a 4-subunit (alpha/beta/gamma/ferredoxin) version of the system. This alpha/gamma plus beta structure was used to define the set of sequences to include in this model.

Date:

2020-03-25