Protein Family Models

This family of proteins participate in the biosynthesis of 7-carboxy-7-deazaguanine. They catalyse the conversion of 7-deaza-7-carboxyguanine to preQ0 [1-3]. [1]. 14660578. Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine. Reader JS, Metzgar D, Schimmel P, de Crecy-Lagard V;. J Biol Chem. 2004;279:6280-6285. [2]. 16199558. Genetic analysis identifies a function for the queC (ybaX) gene product at an initial step in the queuosine biosynthetic pathway in Escherichia coli. Gaur R, Varshney U;. J Bacteriol. 2005;187:6893-6901. [3]. 19354300. The deazapurine biosynthetic pathway revealed: in vitro enzymatic synthesis of PreQ(0) from guanosine 5'-triphosphate in four steps. McCarty RM, Somogyi A, Lin G, Jacobsen NE, Bandarian V;. Biochemistry. 2009;48:3847-3852. [4]. 18491386. Crystal structure of QueC from Bacillus subtilis: an enzyme involved in preQ1 biosynthesis. Cicmil N, Huang RH;. Proteins. 2008;72:1084-1088. (from Pfam)

Date:

2024-10-16

ThiI is required for thiazole synthesis, required for thiamine biosynthesis [1]. [1]. 9209060. Characterization of thiI, a new gene involved in thiazole biosynthesis in Salmonella typhimurium. Webb E, Claas K, Downs DM;. J Bacteriol 1997;179:4399-4402. (from Pfam)

Date:

2024-10-16

Most members of this family are asparagine synthase, which catalyzes conversion of aspartate to asparagine. Exceptions that score well against the HMM include isopeptide bond formation proteins for lasso peptide biosynthesis. But note that many additional proteins, such as the 7-cyano-7-deazaguanine synthase QueC, hit with scores barely above the model's cutoff scores to N-terminal regions about 60 amino acids long, whereas the full length of the model is 355.

Date:

2024-08-14

Members of this protein family are QueC, involved in synthesizing pre-Q0 from GTP en route to tRNA modification with queuosine. This protein family is represented by a single member in nearly every completed large (> 1000 genes) prokaryotic genome. In Rhizobium meliloti, the gene was designated exsB, possibly because of polar effects on exsA expression in a shared polycistronic mRNA. In Arthrobacter viscosus, the homologous gene was designated ALU1 and was associated with an aluminum tolerance phenotype.

Gene:

queC

Date:

2021-04-27