Protein Family Models

This entry represents the C-terminal domain found in transketolase and transketolase-like enzymes [1-5], such as pyruvate dehydrogenase E1 component (OPD1) [4]. Paper describing PDB structure 1ay0. [1]. 9398292. Identification of catalytically important residues in yeast transketolase. Wikner C, Nilsson U, Meshalkina L, Udekwu C, Lindqvist Y, Schneider G;. Biochemistry. 1997;36:15643-15649. Paper describing PDB structure 1gpu. [2]. 11773632. Snapshot of a key intermediate in enzymatic thiamin catalysis: crystal structure of the alpha-carbanion of (alpha,beta-dihydroxyethyl)-thiamin diphosphate in the active site of transketolase from Saccharomyces cerevisiae. Fiedler E, Thorell S, Sandalova T, Golbik R, Konig S, Schneider G;. Proc Natl Acad Sci U S A. 2002;99:591-595. Paper describing PDB structure 1itz. [3]. 12913150. Structure and properties of an engineered transketolase from maize. Gerhardt S, Echt S, Busch M, Freigang J, Auerbach G, Bader G, Martin WF, Bacher A, Huber R, Fischer M;. Plant Physiol. 2003;132:1941-1949. Paper describing PDB structure 1l8a. [4]. 11955070. Structure of the pyruvate dehydrogenase multienzyme complex E1 component from Escherichia coli at 1.85 A resolution. Arjunan P, Nemeria N, Brunskill A, Chandrasekhar K, Sax M, Yan Y, Jordan F, Guest JR, Furey W;. Biochemistry. 2002;41:5213-5221. Paper describing PDB structure 1r9j. [5]. 15149284. Transketolase from Leishmania mexicana has a dual subcellular localization. Veitch NJ, Maugeri DA, Cazzulo JJ, Lindqvist Y, Barrett MP;. Biochem J. 2004;382:759-767. (from Pfam)

Date:

2024-10-16

The C-terminal domain of transketolase has been proposed as a regulatory molecule binding site [2]. [1]. 8176731. Refined structure of transketolase from Saccharomyces cerevisiae at 2.0 A resolution. Nikkola M, Lindqvist Y, Schneider G;. J Mol Biol 1994;238:387-404. [2]. 1628611. Three-dimensional structure of transketolase, a thiamine diphosphate dependent enzyme, at 2.5 A resolution. Lindqvist Y, Schneider G, Ermler U, Sundstrom M;. EMBO J 1992;11:2373-2379. (from Pfam)

Date:

2024-10-16

This family includes transketolase enzymes, pyruvate dehydrogenases, and branched chain alpha-keto acid decarboxylases. [1]. 8176731. Refined structure of transketolase from Saccharomyces cerevisiae at 2.0 A resolution. Nikkola M, Lindqvist Y, Schneider G;. J Mol Biol 1994;238:387-404. [2]. 1628611. Three-dimensional structure of transketolase, a thiamine diphosphate dependent enzyme, at 2.5 A resolution. Lindqvist Y, Schneider G, Ermler U, Sundstrom M;. EMBO J 1992;11:2373-2379. (from Pfam)

Date:

2024-10-16

This family includes transketolase enzymes EC:2.2.1.1. and also partially matches to 2-oxoisovalerate dehydrogenase beta subunit Swiss:P37941 EC:1.2.4.4. Both these enzymes utilise thiamine pyrophosphate as a cofactor, suggesting there may be common aspects in their mechanism of catalysis. [1]. 8176731. Refined structure of transketolase from Saccharomyces cerevisiae at 2.0 A resolution. Nikkola M, Lindqvist Y, Schneider G;. J Mol Biol 1994;238:387-404. [2]. 1628611. Three-dimensional structure of transketolase, a thiamine diphosphate dependent enzyme, at 2.5 A resolution. Lindqvist Y, Schneider G, Ermler U, Sundstrom M;. EMBO J 1992;11:2373-2379. (from Pfam)

Date:

2024-10-16

transketolase family protein such as transketolase, which catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate

Date:

2024-05-21

This HMM is designed to capture orthologs of bacterial transketolases. The group includes two from the yeast Saccharomyces cerevisiae but excludes dihydroxyactetone synthases (formaldehyde transketolases) from various yeasts and the even more distant mammalian transketolases. Among the family of thiamine diphosphate-dependent enzymes that includes transketolases, dihydroxyacetone synthases, pyruvate dehydrogenase E1-beta subunits, and deoxyxylulose-5-phosphate synthases, mammalian and bacterial transketolases seem not to be orthologous.

Gene:

tkt

Date:

2024-07-09