Protein Family Models

Thymidylate synthase complementing protein (Thy1) complements the thymidine growth requirement of the organisms in which it is found, but shows no homology to thymidylate synthase. The bacterial members of this family at least are flavin-dependent thymidylate synthases [2,3,4]. [1]. 9665876. Complete genome sequence of Treponema pallidum, the syphilis spirochete [see comments]. Fraser CM, Norris SJ, Weinstock GM, White O, Sutton GG, Dodson R, Gwinn M, Hickey EK, Clayton R, Ketchum KA, Sodergren E, Hardham JM, McLeod MP, Salzberg S, Peterson J, Khalak H, Richardson D, Howell JK, Chidambaram M, Utterback T, McDonald L, Artiach P,. Science 1998;281:375-388. [2]. 12029065. An alternative flavin-dependent mechanism for thymidylate synthesis. Myllykallio H, Lipowski G, Leduc D, Filee J, Forterre P, Liebl U;. Science. 2002;297:105-107. [3]. 15046578. Two distinct pathways for thymidylate (dTMP) synthesis in (hyper)thermophilic Bacteria and Archaea. Leduc D, Graziani S, Meslet-Cladiere L, Sodolescu A, Liebl U, Myllykallio H;. Biochem Soc Trans. 2004;32:231-235. [4]. 17890305. Flavin-dependent thymidylate synthase ThyX activity: implications for the folate cycle in bacteria. Leduc D, Escartin F, Nijhout HF, Reed MC, Liebl U, Skouloubris S, Myllykallio H;. J Bacteriol. 2007;189:8537-8545. (from Pfam)

Date:

2024-10-16

FAD-dependent thymidylate synthase catalyzes the formation of dTMP and tetrahydrofolate from dUMP and methylenetetrahydrofolate using a flavin coenzyme as the source of reducing equivalents, derived from NADPH

Date:

2016-02-26

Two forms of microbial thymidylate synthase are known: ThyA (2.1.1.45) and ThyX (2.1.1.148). This model describes ThyX, a homotetrameric flavoprotein. Both enzymes convert dUMP to dTMP. Under oxygen-limiting conditions, thyX can complement a thyA mutation.

Gene:

thyX

Date:

2024-05-29