Protein Family Models

This domain is found at the C-terminal end of the putative pilin protein from Clostridioides difficile (PilW) and similar sequences mainly found in Clostidia, which plays a role in biofilm formation. PilW shows a single transmembrane helix followed by a soluble domain and a central alpha-helix with a beta-sheet packed against it [1]. This long domain is found associated with Pfam:PF07963. Paper describing PDB structure 4ogm. [1]. 25599642. Structural and evolutionary analyses show unique stabilization strategies in the type IV pili of Clostridium difficile. Piepenbrink KH, Maldarelli GA, Martinez de la Pena CF, Dingle TC, Mulvey GL, Lee A, von Rosenvinge E, Armstrong GD, Donnenberg MS, Sundberg EJ;. Structure. 2015;23:385-396. (from Pfam)

Date:

2024-10-16

This short motif directs methylation of the conserved phenylalanine residue. It is most often found at the N-terminus of pilins and other proteins involved in secretion, see Pfam:PF00114, Pfam:PF05946, Pfam:PF02501 and Pfam:PF07596. (from Pfam)

Date:

2024-08-14

type II secretion system protein similar to Pseudomonas aeruginosa type II secretion system protein H, which is involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins; required for the translocation of a variety of enzymes across the outer membrane; pilin similar to fimbrial protein PilE, which is required for pilus biogenesis, as well as fimbrial protein PilA, which is responsible for the adhesive property of the pilus

Date:

2024-08-08

This model describes many but not all examples of the N-terminal region of bacterial proteins that resemble type IV pilins at their N-terminus, with a cleavage site G^FxxxE followed by a hydrophobic stretch. The new N-terminal residue, usually Phe, is methylated. Separate domains of the prepilin peptidase appear responsible for cleavage and methylation. Proteins with this N-terminal region include type IV pilins and other components of pilus biogenesis, competence proteins, and type II secretion proteins. Typically several proteins in a single operon have this N-terminal domain. The N-terminal cleavage and methylation site is described by PROSITE motif PS00409 as [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14).

Date:

2019-09-10