Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction [3]. This family contains transposases for IS4 Swiss:P03835 [1], IS421 Swiss:P11901 [2], IS5377 Swiss:Q45620, IS427 [4], IS402 [5], IS1355 Swiss:O69604, IS5, which was original isolated in bacteriophage lambda [6]. [1]. 6268937. The sequence of IS4. Klaer R, Kuhn S, Tillmann E, Fritz HJ, Starlinger P;. Mol Gen Genet 1981;181:169-175. [2]. 2542093. IS421, a new insertion sequence in Escherichia coli. Sato S, Nakada Y, Shiratsuchi A;. FEBS Lett 1989;249:21-26. [3]. 8335077. Identification and analysis of the gas vesicle gene cluster on an unstable plasmid of Halobacterium halobium. DasSarma S;. Plasmid 1993;29:1-9. [4]. 10207011. The three-dimensional structure of a Tn5 transposase-related protein determined to 2.9-A resolution. Davies DR, Braam LM, Reznikoff WS, Rayment I;. J Biol Chem 1999;274:11904-11913. [5]. 1963949. Nucleotide sequence analysis of IS427 and its target sites in Agrobacterium tumefaciens T37. De Meirsman C, Van Soom C, Verreth C, Van Gool A, Vanderleyden J;. Plasmid 1990;24:227-234. [6]. 1650732. Nucleotide sequence of IS402 from Pseudomonas cepacia. Ferrante AA, Lessie TG;. Gene 1991;102:143-144. [7]. 6281654. Structural analysis of insertion sequence IS5. Kroger M, Hobom G;. Nature 1982;297:159-162. (from Pfam)
GO Terms:- Date:
- 2024-10-16