Protein Family Models

This region is found in many but not all ATP-dependent DNA ligase enzymes (EC:6.5.1.1). It is thought to be involved in DNA binding and in catalysis. In human DNA ligase I (Swiss:P18858), and in Saccharomyces cerevisiae (Swiss:P04819), this region was necessary for catalysis, and separated from the amino terminus by targeting elements. In vaccinia virus (Swiss:P16272) this region was not essential for catalysis, but deletion decreases the affinity for nicked DNA and decreased the rate of strand joining at a step subsequent to enzyme-adenylate formation [1]. [1]. 9016621. Domain structure of vaccinia DNA ligase. Sekiguchi J, Shuman S;. Nucleic Acids Res 1997;25:727-734. (from Pfam)

Date:

2024-10-16

This region is found in many but not all ATP-dependent DNA ligase enzymes (EC:6.5.1.1). It is thought to constitute part of the catalytic core of ATP dependent DNA ligase [1]. [1]. 9016621. Domain structure of vaccinia DNA ligase. Sekiguchi J, Shuman S;. Nucleic Acids Res 1997;25:727-734. (from Pfam)

Date:

2024-10-16

This domain belongs to a more diverse superfamily, including Pfam:PF01331 and Pfam:PF01653 [3]. [1]. 8653795. Crystal structure of an ATP-dependent DNA ligase from bacteriophage T7. Subramanya HS, Doherty AJ, Ashford SR, Wigley DB;. Cell 1996;85:607-615. [2]. 9363683. Mammalian DNA ligases. Tomkinson AE, Levin DS;. Bioessays 1997;19:893-901. [3]. 10222208. Gleaning non-trivial structural, functional and evolutionary information about proteins by iterative database searches. Aravind L, Koonin EV;. J Mol Biol 1999;287:1023-1040. (from Pfam)

Date:

2024-10-16

Catalyzes the ATP-dependent formation of a phosphodiester at the site of a single strand break in duplex DNA

Date:

2021-08-25

This HMM describes a family of ATP-dependent DNA ligases present in about 12 % of prokaryotic genomes. It occurs as part of a four-gene system with an exonuclease, a helicase and a phosphoesterase, with all four genes clustered or at least the first two and last two paired. This family resembles DNA ligase I (see TIGR00574 and PF01068), and its presumed function may be in DNA repair, replication, or recombination.

Date:

2021-09-20

ATP-dependent DNA ligase catalyzes the joining of breaks in the phosphodiester backbone of duplex DNA

Date:

2021-02-02