Protein Family Models

This family represents the N-terminal region of proteins carrying the transposase enzyme, DDE_Tnp_1 (that was Transposase_11), Pfam:PF01609, at the C-terminus. The full-length members are Insertion Element 4, IS4. Within the collection of E.coli strains, ECOR, the number of IS4 elements varies from zero to 14, with an average of 5 copies/strain [1]. [1]. 2996976. Joint distribution of insertion elements IS4 and IS5 in natural. isolates of Escherichia coli.. Dykhuizen DE, Sawyer SA, Green L, Miller RD, Hartl DL;. Genetics. 1985;111:219-231. (from Pfam)

Date:

2024-08-14

Transposase proteins are necessary for efficient DNA transposition. This domain is a member of the DDE superfamily, which contain three carboxylate residues that are believed to be responsible for coordinating metal ions needed for catalysis. The catalytic activity of this enzyme involves DNA cleavage at a specific site followed by a strand transfer reaction [3]. This family contains transposases for IS4 Swiss:P03835 [1], IS421 Swiss:P11901 [2], IS5377 Swiss:Q45620, IS427 [4], IS402 [5], IS1355 Swiss:O69604, IS5, which was original isolated in bacteriophage lambda [6]. [1]. 6268937. The sequence of IS4.. Klaer R, Kuhn S, Tillmann E, Fritz HJ, Starlinger P;. Mol Gen Genet 1981;181:169-175.. [2]. 2542093. IS421, a new insertion sequence in Escherichia coli.. Sato S, Nakada Y, Shiratsuchi A;. FEBS Lett 1989;249:21-26.. [3]. 8335077. Identification and analysis of the gas vesicle gene cluster on. an unstable plasmid of Halobacterium halobium.. DasSarma S;. Plasmid 1993;29:1-9.. [4]. 10207011. The three-dimensional structure of a Tn5 transposase-related. protein determined to 2.9-A resolution.. Davies DR, Braam LM, Reznikoff WS, Rayment I;. J Biol Chem 1999;274:11904-11913.. [5]. 1963949. Nucleotide sequence analysis of IS427 and its target sites in. Agrobacterium tumefaciens T37.. De Meirsman C, Van Soom C, Verreth C, Van Gool A, Vanderleyden. J;. Plasmid 1990;24:227-234.. [6]. 1650732. Nucleotide sequence of IS402 from Pseudomonas cepacia.. Ferrante AA, Lessie TG;. Gene 1991;102:143-144.. [7]. 6281654. Structural analysis of insertion sequence IS5.. Kroger M, Hobom G;. Nature 1982;297:159-162. (from Pfam)

Date:

2024-08-14

transposase binds to the end of a transposon and catalyzes the movement of the transposon to another part of the genome by a cut and paste mechanism or a replicative transposition mechanism

Date:

2019-01-30

Date:

2021-03-03

Date:

2019-09-10