Protein Family Models

The full-length members of this family, eg Swiss:P53816, are representatives of a novel class II tumour-suppressor family, designated as H-REV107-like. This domain is the catalytic N-terminal proline-rich region of the protein. The downstream region is a putative C-terminal transmembrane domain which is found to be crucial for cellular localisation, but not necessary for the enzyme activity [1]. H-REV107-like proteins are homologous to lecithin retinol acyltransferase (LRAT), an enzyme that catalyses the transfer of the sn-1 acyl group of phosphatidylcholine to all-trans-retinol and forming a retinyl ester [2]. [1]. 20837014. Solution structure of the N-terminal catalytic domain of human H-REV107--a novel circular permutated NlpC/P60 domain. Ren X, Lin J, Jin C, Xia B;. FEBS Lett. 2010;584:4222-4226. [2]. 3410848. Evidence for a lecithin-retinol acyltransferase activity in the rat small intestine. MacDonald PN, Ong DE;. J Biol Chem. 1988;263:12478-12482. (from Pfam)

Date:

2024-10-16

This family includes PspA a protein that suppresses sigma54-dependent transcription. The PspA protein, a negative regulator of the Escherichia coli phage shock psp operon, is produced when virulence factors are exported through secretins in many Gram-negative pathogenic bacteria and its homologue in plants, VIPP1, plays a critical role in thylakoid biogenesis, essential for photosynthesis. Activation of transcription by the enhancer-dependent bacterial sigma(54) containing RNA polymerase occurs through ATP hydrolysis-driven protein conformational changes enabled by activator proteins that belong to the large AAA(+) mechanochemical protein family. It has been shown that PspA directly and specifically acts upon and binds to the AAA(+) domain of the PspF transcription activator [2]. [1]. 10629175. The PspA protein of Escherichia coli is a negative regulator of sigma(54)-dependent transcription. Dworkin J, Jovanovic G, Model P;. J Bacteriol 2000;182:311-319. [2]. 12079332. Mechanism of Action of the Escherichia coli Phage Shock Protein PspA in Repression of the AAA Family Transcription Factor PspF. Elderkin S, Jones S, Schumacher J, Studholme D, Buck M;. J Mol Biol 2002;320:23-37. (from Pfam)

Date:

2024-10-16