Protein Family Models

This is the HNH endonuclease domain found in a set of group II intron proteins, including AIM1/2 from yeast and its orthologue from S. pombe. This domain is also found in related bacterial sequences [1]. Paper describing PDB structure 5g2x. [1]. 27136327. Structure of a group II intron in complex with its reverse transcriptase. Qu G, Kaushal PS, Wang J, Shigematsu H, Piazza CL, Agrawal RK, Belfort M, Wang HW;. Nat Struct Mol Biol. 2016;23:549-557. (from Pfam)

Date:

2024-10-16

Group II introns use intron-encoded reverse transcriptase, maturase and DNA endonuclease activities for site-specific insertion into DNA [2]. Although this type of intron is self splicing in vitro they require a maturase protein for splicing in vivo. It has been shown that a specific region of the aI2 intron is needed for the maturase function [1]. This region was found to be conserved in group II introns and called domain X [3]. [1]. 8029012. Splicing defective mutants of the COXI gene of yeast mitochondrial DNA: initial definition of the maturase domain of the group II intron aI2. Moran JV, Mecklenburg KL, Sass P, Belcher SM, Mahnke D, Lewin A, Perlman P;. Nucleic Acids Res 1994;22:2057-2064. [2]. 9362497. Group II intron endonucleases use both RNA and protein subunits for recognition of specific sequences in double-stranded DNA. Guo H, Zimmerly S, Perlman PS, Lambowitz AM;. EMBO J 1997;16:6835-6848. [3]. 8255751. Evolutionary relationships among group II intron-encoded proteins and identification of a conserved domain that may be related to maturase function. Mohr G, Perlman PS, Lambowitz AM;. Nucleic Acids Res 1993;21:4991-4997. (from Pfam)

Date:

2024-10-16

A reverse transcriptase gene is usually indicative of a mobile element such as a retrotransposon or retrovirus. Reverse transcriptases occur in a variety of mobile elements, including retrotransposons, retroviruses, group II introns, bacterial msDNAs, hepadnaviruses, and caulimoviruses. [1]. 1698615. Origin and evolution of retroelements based upon their reverse transcriptase sequences. Xiong Y, Eickbush TH;. EMBO J 1990;9:3353-3362. (from Pfam)

Date:

2024-10-16

reverse transcriptase/maturase family protein similar to reverse transcriptases with group II intron origin that transcribe DNA using RNA as template

Date:

2020-10-19