Protein Family Models

This entry includes small conductance mechanosensitive channels (MscS), which assembles to a homoheptameric complex [1-6]. Each monomer consists of a N-terminal transmembrane region and a large cytoplasmic segment composed of a five-stranded antiparallel beta-sheet (middle/second) domain and a C-terminal alpha/beta-domain [4-7]. The N-terminal region consists of three membrane spanning helices (TM1, TM2 and TM3), TM2 and TM3 are represented in this entry. In the heptamer, the permeation pathway is formed by the packing of TM3s, flanked by TM1-TM2 helical hairpins [4]. [1]. 11296222. Structural and functional differences between two homologous mechanosensitive channels of Methanococcus jannaschii. Kloda A, Martinac B;. EMBO J 2001;20:1888-1896. [2]. 11159397. Molecular identification of a mechanosensitive channel in archaea. Kloda A, Martinac B;. Biophys J 2001;80:229-240. [3]. 11275684. Mechanosensitive channels in prokaryotes. Martinac B;. Cell Physiol Biochem 2001;11:61-76. [4]. 23339071. Open and shut: crystal structures of the dodecylmaltoside solubilized mechanosensitive channel of small conductance from Escherichia coli and Helicobacter pylori at 4.4 A and 4.1 A resolutions. Lai JY, Poon YS, Kaiser JT, Rees DC;. Protein Sci. 2013;22:502-509. [5]. 23074248. Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance. Zhang X, Wang J, Feng Y, Ge J, Li W, Sun W, Iscla I, Yu J, Blount P, Li Y, Yang M;. Proc Natl Acad Sci U S A. 2012;109:18180-18185. [6]. 23012406. Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double re. TRUNCATED at 1650 bytes (from Pfam)

Date:

2024-10-16

This entry includes small conductance mechanosensitive channels (MscS), which assembles to a homoheptameric complex [1-6]. Each monomer consists of a N-terminal transmembrane region and a large cytoplasmic segment composed of a five-stranded antiparallel beta-sheet domain (beta-domain, Pfam:PF00924) and a C-terminal alpha/beta-domain [1,2,3]. This is the C-terminal domain of McsS which, together with the middle beta-domain, creates a framework that connects to the cytoplasm through distinct openings [4-6]. [1]. 11296222. Structural and functional differences between two homologous mechanosensitive channels of Methanococcus jannaschii. Kloda A, Martinac B;. EMBO J 2001;20:1888-1896. [2]. 11159397. Molecular identification of a mechanosensitive channel in archaea. Kloda A, Martinac B;. Biophys J 2001;80:229-240. [3]. 11275684. Mechanosensitive channels in prokaryotes. Martinac B;. Cell Physiol Biochem 2001;11:61-76. [4]. 34376558. Mechanosensitive channel gating by delipidation. Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B;. Proc Natl Acad Sci U S A. 2021; [Epub ahead of print]. [5]. 23339071. Open and shut: crystal structures of the dodecylmaltoside solubilized mechanosensitive channel of small conductance from Escherichia coli and Helicobacter pylori at 4.4 A and 4.1 A resolutions. Lai JY, Poon YS, Kaiser JT, Rees DC;. Protein Sci. 2013;22:502-509. [6]. 23074248. Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance. Zhang X, Wang J, Feng Y, Ge J, Li W, Sun. TRUNCATED at 1650 bytes (from Pfam)

Date:

2024-10-16

The small mechanosensitive channel, MscS, is a part of the turgor-driven solute efflux system that protects bacteria from lysis in the event of osmotic shock. The MscS protein alone is sufficient to form a functional mechanosensitive channel gated directly by tension in the lipid bilayer. The MscS proteins are heptamers of three transmembrane subunits with seven converging M3 domains, and this domain is one of the inner membrane domains. [1]. 10202137. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR;. EMBO J. 1999;18:1730-1737. [2]. 12080120. Purification of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes. Sukharev S;. Biophys J. 2002;83:290-298. [3]. 16079835. A possible unifying principle for mechanosensation. Kung C;. Nature. 2005;436:647-654. (from Pfam)

Date:

2024-10-16

The small mechanosensitive channel, MscS, is a part of the turgor-driven solute efflux system that protects bacteria from lysis in the event of osmotic shock. The MscS protein alone is sufficient to form a functional mechanosensitive channel gated directly by tension in the lipid bilayer. The MscS proteins are heptamers of three transmembrane subunits with seven converging M3 domains, and this MscS_porin is towards the N-terminal of the molecules. The high concentration of negative charges at the extracellular entrance of the pore helps select the cations for efflux. [1]. 10202137. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. Levina N, Totemeyer S, Stokes NR, Louis P, Jones MA, Booth IR;. EMBO J. 1999;18:1730-1737. [2]. 12080120. Purification of the small mechanosensitive channel of Escherichia coli (MscS): the subunit structure, conduction, and gating characteristics in liposomes. Sukharev S;. Biophys J. 2002;83:290-298. [3]. 16079835. A possible unifying principle for mechanosensation. Kung C;. Nature. 2005;436:647-654. (from Pfam)

Date:

2024-10-16

This entry includes small conductance mechanosensitive channels (MscS), which assembles to a homoheptameric complex [1-6]. Each monomer consists of a N-terminal transmembrane region and a large cytoplasmic segment composed of a five-stranded antiparallel beta-sheet (middle/second) domain and a C-terminal alpha/beta-domain [4-7]. This is the beta domain which adopts a SH3-like barrel structure. [1]. 11296222. Structural and functional differences between two homologous mechanosensitive channels of Methanococcus jannaschii. Kloda A, Martinac B;. EMBO J 2001;20:1888-1896. [2]. 11159397. Molecular identification of a mechanosensitive channel in archaea. Kloda A, Martinac B;. Biophys J 2001;80:229-240. [3]. 11275684. Mechanosensitive channels in prokaryotes. Martinac B;. Cell Physiol Biochem 2001;11:61-76. [4]. 34376558. Mechanosensitive channel gating by delipidation. Flegler VJ, Rasmussen A, Borbil K, Boten L, Chen HA, Deinlein H, Halang J, Hellmanzik K, Loffler J, Schmidt V, Makbul C, Kraft C, Hedrich R, Rasmussen T, Bottcher B;. Proc Natl Acad Sci U S A. 2021; [Epub ahead of print]. [5]. 23012406. Conformational state of the MscS mechanosensitive channel in solution revealed by pulsed electron-electron double resonance (PELDOR) spectroscopy. Pliotas C, Ward R, Branigan E, Rasmussen A, Hagelueken G, Huang H, Black SS, Booth IR, Schiemann O, Naismith JH;. Proc Natl Acad Sci U S A. 2012;109:E2675-E2682. [6]. 23339071. Open and shut: crystal structures of the dodecylmaltoside solubilized mechanosensitive channel of small conductance from Escherichia coli and Helicobacter pylori at 4.4 A and 4.1 A resolutions. Lai JY, Poon YS, K. TRUNCATED at 1650 bytes (from Pfam)

Date:

2024-10-16

miniconductance mechanosensitive channel MscM is a mechanosensitive channel that protects cells against hypoosmotic stress when highly overexpressed

Date:

2019-06-07

Gene:

mscM

Date:

2021-08-26