Protein Family Models

RNA polymerases catalyse the DNA dependent polymerisation of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain, domain 3, represents the pore domain. The 3' end of RNA is positioned close to this domain. The pore delimited by this domain is thought to act as a channel through which nucleotides enter the active site and/or where the 3' end of the RNA may be extruded during back-tracking [1,2]. [1]. 8910400. Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase. Severinov K, Mustaev A, Kukarin A, Muzzin O, Bass I, Darst SA, Goldfarb A;. J Biol Chem 1996;271:27969-27974. [2]. 11313498. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Cramer P, Bushnell DA, Kornberg RD;. Science 2001;292:1863-1876. (from Pfam)

Date:

2024-10-16

RNA polymerases catalyse the DNA dependent polymerisation of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain, domain 4, represents the funnel domain. The funnel contain the binding site for some elongation factors [1,2]. [1]. 8910400. Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase. Severinov K, Mustaev A, Kukarin A, Muzzin O, Bass I, Darst SA, Goldfarb A;. J Biol Chem 1996;271:27969-27974. [2]. 11313498. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Cramer P, Bushnell DA, Kornberg RD;. Science 2001;292:1863-1876. (from Pfam)

Date:

2024-10-16

RNA polymerases catalyse the DNA dependent polymerisation of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain, domain 5, represents the discontinuous cleft domain that is required to from the central cleft or channel where the DNA is bound [1,2]. [1]. 8910400. Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase. Severinov K, Mustaev A, Kukarin A, Muzzin O, Bass I, Darst SA, Goldfarb A;. J Biol Chem 1996;271:27969-27974. [2]. 11313498. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Cramer P, Bushnell DA, Kornberg RD;. Science 2001;292:1863-1876. (from Pfam)

Date:

2024-10-16

RNA polymerases catalyse the DNA dependent polymerisation of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain, domain 1, represents the clamp domain, which a mobile domain involved in positioning the DNA, maintenance of the transcription bubble and positioning of the nascent RNA strand [1,2]. [1]. 8910400. Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase. Severinov K, Mustaev A, Kukarin A, Muzzin O, Bass I, Darst SA, Goldfarb A;. J Biol Chem 1996;271:27969-27974. [2]. 11313498. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Cramer P, Bushnell DA, Kornberg RD;. Science 2001;292:1863-1876. (from Pfam)

Date:

2024-10-16

RNA polymerases catalyse the DNA dependent polymerisation of RNA. Prokaryotes contain a single RNA polymerase compared to three in eukaryotes (not including mitochondrial. and chloroplast polymerases). This domain, domain 2, contains the active site. The invariant motif -NADFDGD- binds the active site magnesium ion [1,2]. [1]. 8910400. Structural modules of the large subunits of RNA polymerase. Introducing archaebacterial and chloroplast split sites in the beta and beta' subunits of Escherichia coli RNA polymerase. Severinov K, Mustaev A, Kukarin A, Muzzin O, Bass I, Darst SA, Goldfarb A;. J Biol Chem 1996;271:27969-27974. [2]. 11313498. Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Cramer P, Bushnell DA, Kornberg RD;. Science 2001;292:1863-1876. (from Pfam)

Date:

2024-10-16

DNA-directed RNA polymerase (RNAP) subunit beta' is part of the RNAP catalytic core that catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Date:

2024-05-15

Bacteria have a single DNA-directed RNA polymerase, with required subunits that include alpha, beta, and beta-prime. This model describes the predominant architecture of the beta-prime subunit in most bacteria. This model excludes from among the bacterial mostly sequences from the cyanobacteria, where RpoC is replaced by two tandem genes homologous to it but also encoding an additional domain.

Gene:

rpoC

Date:

2024-05-14