Protein Family Models

This domain is found at the N-terminal end of Glycosyltransferase TibC from Escherichia coli and similar bacterial. TibC is an autotransporter adhesin heptosyltransferase (AAH) homologue that catalyses the heptosylation of the autotransporters AIDA-I/TibA and confers AIDA-I-mediated bacterial adhesion to host cells. This domain adopts a beta-barrel fold with two four-stranded sheets that pack against each other [1,2]. Paper describing PDB structure 4rap. [1]. 25310236. A structural mechanism for bacterial autotransporter glycosylation by a dodecameric heptosyltransferase family. Yao Q, Lu Q, Wan X, Song F, Xu Y, Hu M, Zamyatina A, Liu X, Huang N, Zhu P, Shao F;. Elife. 2014; [Epub ahead of print]. [2]. 11953358. Functional substitution of the TibC protein of enterotoxigenic Escherichia coli strains for the autotransporter adhesin heptosyltransferase of the AIDA system. Moormann C, Benz I, Schmidt MA;. Infect Immun. 2002;70:2264-2270. (from Pfam)

Date:

2024-10-16

Members of this family belong to glycosyltransferase family 9 [1]. Lipopolysaccharide is a major component of the outer leaflet of the outer membrane in Gram-negative bacteria. It is composed of three domains; lipid A, Core oligosaccharide and the O-antigen. All of these enzymes transfer heptose to the lipopolysaccharide core. [1]. 9334165. A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Campbell JA, Davies GJ, Bulone V, Henrissat B;. Biochem J 1997;326:929-939. [2]. 9446588. Enzymatic synthesis of lipopolysaccharide in Escherichia coli. Purification and properties of heptosyltransferase I. Kadrmas JL, Raetz CR;. J Biol Chem 1998;273:2799-2807. (from Pfam)

Date:

2024-10-16

Both Aah (autotransporter adhesin heptosyltransferase) and TibC (tib is enterotoxigenic invasion locus B) are protein O-heptosyltransferases that act on multiple sites from repeat regions of proteins exported by autotransporters. Aah glycosylates AIDA, or autotransporter adhesin involved in diffuse adherence, TibC acts on TibA, but TibC can replace Aah.

Date:

2022-01-26

glycosyltransferase family protein may synthesize oligosaccharides, polysaccharides, and glycoconjugates by transferring the sugar moiety from an activated nucleotide-sugar donor to an acceptor molecule, which may be a growing oligosaccharide, a lipid, or a protein| glycosyltransferase family 4 (GT4) protein catalyzes the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds

Date:

2017-03-02