Protein Family Models

This family includes ecdysteroid 22-kinase, an enzyme responsible for the phosphorylation of ecdysteroids (insect growth and moulting hormones) at C-22, to form physiologically inactive ecdysteroid 22-phosphates [1]. Most insects contain 12 to 105 genes encoding this family and yet so far only one enzyme (ecdysteroid 22-kinase from Bombyx mori) has characterised substrates (2-deoxyecdysone, ecdysone, 20-hydroxyecdysone). There are good reasons to believe that this family includes kinases that act on other small molecule substrates and that they may function in detoxification processes [2]. [1]. 16899460. Purification, kinetic characterization, and molecular cloning of a novel enzyme, ecdysteroid 22-kinase. Sonobe H, Ohira T, Ieki K, Maeda S, Ito Y, Ajimura M, Mita K, Matsumoto H, Wilder MN;. J Biol Chem. 2006;281:29513-29524. [2]. 32540344. Genomic and transcriptomic analyses in Drosophila suggest that the ecdysteroid kinase-like (EcKL) gene family encodes the 'detoxification-by-phosphorylation' enzymes of insects. Scanlan JL, Gledhill-Smith RS, Battlay P, Robin C;. Insect Biochem Mol Biol. 2020; [Epub ahead of print] (from Pfam)

Date:

2024-10-16

This family consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include: aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation [2]. This family also includes homoserine kinase. This family is related to fructosamine kinase Pfam:PF03881. [1]. 2163618. Cloning of aminoglycoside phosphotransferase (APH) gene from antibiotic- producing strain of Bacillus circulans into a high-expression vector, pKK223-3. Purification, properties and location of the enzyme [published erratum appears in Biochem J 1991 Feb 1. Sarwar M, Akhtar M;. Biochem J 1990;268:671-677. [2]. 2167474. PCR cloning of a streptomycin phosphotransferase (aphE) gene from Streptomyces griseus ATCC 12475. Trower MK, Clark KG;. Nucleic Acids Res 1990;18:4615-4615. (from Pfam)

Date:

2024-10-16

Choline kinase catalyses the committed step in the synthesis of phosphatidylcholine by the CDP-choline pathway [1]. This alignment covers the protein kinase portion of the protein. The divergence of this family makes it very difficult to create a model that specifically predicts choline/ethanolamine kinases only. However if [add Pfam ID here for Choline_kinase_C] is also present then it is definitely a member of this family. [1]. 9506987. Expression, purification, and characterization of choline kinase, product of the CKI gene from Saccharomyces cerevisiae. Kim KH, Voelker DR, Flocco MT, Carman GM;. J Biol Chem 1998;273:6844-6852. (from Pfam)

Date:

2024-10-16