Bacterial production of D-erythroascorbic acid and L-ascorbic acid through functional expression of Saccharomyces cerevisiae D-arabinono-1,4-lactone oxidase in Escherichia coli

Appl Environ Microbiol. 1999 Oct;65(10):4685-7. doi: 10.1128/AEM.65.10.4685-4687.1999.

Abstract

D-Arabinono-1,4-lactone oxidase, which catalyzes the terminal step in the biosynthesis of D-erythroascorbic acid in Saccharomyces cerevisiae, was functionally expressed in Escherichia coli inherently lacking the enzyme. The recombinant E. coli strain expressing the enzyme could overproduce D-erythroascorbic acid and L-ascorbic acid when supplied with D-arabinono-1,4-lactone and L-galactono-1,4-lactone, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascorbic Acid / biosynthesis*
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Recombinant Proteins / biosynthesis
  • Saccharomyces cerevisiae / enzymology*
  • Sugar Alcohol Dehydrogenases / biosynthesis*

Substances

  • Recombinant Proteins
  • isoascorbic acid
  • Sugar Alcohol Dehydrogenases
  • D-arabinonolactone oxidase
  • Ascorbic Acid