The Arabidopsis abscisic acid response gene ABI5 encodes a basic leucine zipper transcription factor

Plant Cell. 2000 Apr;12(4):599-609. doi: 10.1105/tpc.12.4.599.

Abstract

The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. We isolated the ABI5 gene by using a positional cloning approach and found that it encodes a member of the basic leucine zipper transcription factor family. The previously characterized abi5-1 allele encodes a protein that lacks the DNA binding and dimerization domains required for ABI5 function. Analyses of ABI5 expression provide evidence for ABA regulation, cross-regulation by other ABI genes, and possibly autoregulation. Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Abscisic Acid / pharmacology*
  • Amino Acid Sequence
  • Arabidopsis / drug effects*
  • Arabidopsis / embryology
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis Proteins*
  • Base Sequence
  • Basic-Leucine Zipper Transcription Factors
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Plant / drug effects
  • Genes, Plant / genetics
  • Genetic Complementation Test
  • Leucine Zippers*
  • Molecular Sequence Data
  • Mutation / genetics
  • Plant Proteins / chemistry
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Plants, Genetically Modified
  • Protein Structure, Tertiary
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Seeds / drug effects
  • Seeds / genetics
  • Seeds / growth & development
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription Factors / chemistry*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • ABI5 protein, Arabidopsis
  • Arabidopsis Proteins
  • Basic-Leucine Zipper Transcription Factors
  • DNA-Binding Proteins
  • Plant Proteins
  • RNA, Messenger
  • Transcription Factors
  • Abscisic Acid

Associated data

  • GENBANK/AB023288
  • GENBANK/AC006921
  • GENBANK/AF001453
  • GENBANK/AF001454
  • GENBANK/AF061870
  • GENBANK/P42777
  • GENBANK/U25283