Abstract
The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. We isolated the ABI5 gene by using a positional cloning approach and found that it encodes a member of the basic leucine zipper transcription factor family. The previously characterized abi5-1 allele encodes a protein that lacks the DNA binding and dimerization domains required for ABI5 function. Analyses of ABI5 expression provide evidence for ABA regulation, cross-regulation by other ABI genes, and possibly autoregulation. Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Abscisic Acid / pharmacology*
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Amino Acid Sequence
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Arabidopsis / drug effects*
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Arabidopsis / embryology
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Arabidopsis / genetics*
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Arabidopsis / growth & development
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Arabidopsis Proteins*
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Base Sequence
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Basic-Leucine Zipper Transcription Factors
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Chromosome Mapping
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Cloning, Molecular
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Gene Expression Regulation, Plant / drug effects
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Genes, Plant / genetics
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Genetic Complementation Test
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Leucine Zippers*
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Molecular Sequence Data
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Mutation / genetics
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Plant Proteins / chemistry
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Plant Proteins / genetics
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Plant Proteins / metabolism
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Plants, Genetically Modified
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Protein Structure, Tertiary
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RNA, Messenger / analysis
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RNA, Messenger / genetics
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Seeds / drug effects
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Seeds / genetics
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Seeds / growth & development
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Sequence Alignment
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Sequence Homology, Amino Acid
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Transcription Factors / chemistry*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
Substances
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ABI5 protein, Arabidopsis
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Arabidopsis Proteins
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Basic-Leucine Zipper Transcription Factors
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DNA-Binding Proteins
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Plant Proteins
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RNA, Messenger
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Transcription Factors
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Abscisic Acid
Associated data
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GENBANK/AB023288
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GENBANK/AC006921
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GENBANK/AF001453
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GENBANK/AF001454
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GENBANK/AF061870
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GENBANK/P42777
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GENBANK/U25283