Isolation and sequencing of a gene, C-ADE1, and its use for a host-vector system in Candida maltosa with two genetic markers

Agric Biol Chem. 1991 Jan;55(1):59-65.

Abstract

The host-vector systems of an n-alkane-assimilating-yeast, Candida maltosa, that we previously constructed consisted of a vector replicating with an ARS region of this yeast, and C. maltosa strains J288 (leu2) or CH1 (his5) as hosts. Since each of these hosts has a single genetic marker, we have developed a new host-vector system using two genetic markers. By UV irradiation of strain CH1, an adenine auxotrophic mutant, CHA1, forming red colonies was isolated. A DNA fragment complementing this deficiency was isolated from the C. maltosa genome. Since the DNA fragment also complemented the ade1 mutation of S. cerevisiae, we termed a gene contained in this DNA fragment C-ADE1. The nucleotides of C-ADE1 were sequenced. The deduced amino acid sequence (291 residues) had 65.6% homology with that of ADE1 of S. cerevisiae (306 residues). Having the cloned C-ADE1 DNA, we improved the host-vector system of C. maltosa.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenine / metabolism
  • Amino Acid Sequence
  • Base Sequence
  • Candida / genetics*
  • Candida / metabolism
  • Cloning, Molecular
  • Genes, Fungal*
  • Genetic Markers / genetics
  • Genetic Vectors / genetics*
  • Molecular Sequence Data
  • Mutation
  • Open Reading Frames
  • Restriction Mapping
  • Transformation, Genetic / physiology

Substances

  • Genetic Markers
  • Adenine