The transcription factor, Bright, is not expressed in all human B lymphocyte subpopulations

Cell Immunol. 2004 Mar;228(1):42-53. doi: 10.1016/j.cellimm.2004.03.004.

Abstract

Bright is an ARID family transcription factor that increases immunoglobulin heavy chain transcription. In the mouse, Bright expression is tightly regulated and B cell-restricted and the Bright protein associates with Bruton's tyrosine kinase (Btk), the defective enzyme in X-linked immunodeficiency. Human X-linked agammaglobulinemia results from defects in Btk and leads to early blocks in B lymphocyte development. Because so little is known about human Bright, we sought to determine where human Bright is expressed in normal B cell differentiation and whether it also forms complexes with Btk. Although human and mouse Bright exhibited similar expression patterns in normal B cells, many human transformed B cell lines did not express Bright protein. However, the human protein bound prototypic Bright DNA-binding motifs and, like mouse Bright, was capable of associating with Btk. These data suggest potentially important similarities exist in Bright expression and activity in human and mouse B lymphocytes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Agammaglobulinaemia Tyrosine Kinase
  • B-Lymphocyte Subsets / metabolism*
  • Base Sequence
  • Binding Sites
  • Cell Line, Transformed
  • Consensus Sequence
  • DNA / chemistry
  • DNA / metabolism
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Expression
  • Germinal Center / metabolism
  • Humans
  • Oncogenes / genetics
  • Protein-Tyrosine Kinases / metabolism
  • Stem Cells / metabolism
  • Tissue Distribution
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • ARID3A protein, human
  • DNA-Binding Proteins
  • Trans-Activators
  • Transcription Factors
  • DNA
  • Protein-Tyrosine Kinases
  • Agammaglobulinaemia Tyrosine Kinase
  • BTK protein, human
  • Btk protein, mouse