Structural study of the N-glycans of intercellular adhesion molecule-5 (telencephalin)

Biochim Biophys Acta. 2009 Dec;1790(12):1611-23. doi: 10.1016/j.bbagen.2009.08.012. Epub 2009 Sep 3.

Abstract

Intercellular adhesion molecule-5 (ICAM-5, telencephalin) is a dendritically polarized membrane glycoprotein expressed in tissues distinct from those expressing other ICAMs. Here, we determined the N-glycan structure of ICAM-5 purified from adult rat brain and compared it with that of other ICAMs. N-glycans were released by N-glycosidase F digestion and labeled with p-amino benzoic octylester (ABOE). ABOE-labeled glycans were analyzed by high performance liquid chromatography (HPLC) and mass spectrometry. The N-glycans obtained from rat brain ICAM-5 consisted of approximately 85% neutral, 10.2% sialylated-only, 2.8% sulfated-only, and 1.2% sialylated and sulfated glycans. Compared with the N-glycan structures of human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells, rat brain ICAM-5 had less highly branched glycans, sialylated glycans, and N-acetyllactosamine structures. In contrast, high-mannose-type N-glycans and Lewis X were more commonly found in rat brain ICAM-5 than in human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells. In addition, sulfated glycans contained GlcNAc 6-O-sulfate on the non-reducing terminal side. Our data will be important for the elucidation of the roles of the N-glycans expressed in neural cells, including those present on ICAM-5.

Publication types

  • Comparative Study

MeSH terms

  • 4-Aminobenzoic Acid / chemistry
  • Animals
  • CHO Cells
  • Carbohydrate Sequence
  • Cell Adhesion Molecules / chemistry
  • Cell Adhesion Molecules / metabolism*
  • Chromatography, High Pressure Liquid / methods
  • Cricetinae
  • Cricetulus
  • Humans
  • Molecular Conformation
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / metabolism*
  • Neural Cell Adhesion Molecules / chemistry
  • Neural Cell Adhesion Molecules / metabolism*
  • Neurons / metabolism
  • Polysaccharides / chemistry*
  • Polysaccharides / metabolism
  • Rabbits
  • Rats
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Tandem Mass Spectrometry / methods
  • para-Aminobenzoates

Substances

  • Cell Adhesion Molecules
  • ICAM5 protein, rat
  • Nerve Tissue Proteins
  • Neural Cell Adhesion Molecules
  • Polysaccharides
  • para-Aminobenzoates
  • 4-Aminobenzoic Acid