L-asparaginase II of Escherichia coli K-12: cloning, mapping and sequencing of the ansB gene

Gene. 1990 Jul 2;91(1):101-5. doi: 10.1016/0378-1119(90)90168-q.

Abstract

The Escherichia coli gene ansB, encoding the chemotherapeutic enzyme L-asparaginase II, has been cloned, using a strategy based on the polymerase chain reaction, and sequenced. The amino acid (aa) sequence differs in eleven positions from the data previously derived by direct aa sequencing. A cleavable secretory signal peptide precedes the N terminus of the mature protein. The ansB gene maps to position 3114 kb on the physical map of E. coli [Kohara et al., Cell 50 (1987) 495-508], corresponding to approx. 63.8 min on the genetic map.

MeSH terms

  • Amino Acid Sequence
  • Asparaginase / genetics*
  • Bacteriophage lambda / genetics
  • Base Sequence
  • Chromosomes, Bacterial
  • Cloning, Molecular
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Isoenzymes / genetics*
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Protein Sorting Signals / genetics
  • Restriction Mapping

Substances

  • Isoenzymes
  • Oligonucleotide Probes
  • Protein Sorting Signals
  • Asparaginase

Associated data

  • GENBANK/M34234