Protein phosphatase 1 activity in Drosophila mutants with abnormalities in mitosis and chromosome condensation

FEBS Lett. 1990 Nov 26;275(1-2):39-43. doi: 10.1016/0014-5793(90)81434-p.

Abstract

A Drosophila gene encoding a protein phosphatase 1 (PP1) has been sequenced, and lethal mutations in this locus (87B) analysed. Two mutants (ck19e211 and ck19hs46), which disrupt mitosis, lack the 87B isoenzyme and express only approximately 20% of wild type PP1 activity. The promoter region of the gene is deleted in the ck19e211 mutant. A third mutant (ck19e078), which shows suppression of position effect variegation, but has little effect on mitosis, possesses approximately 35% of wild type PP1 activity. The results indicate that the PP1 87B isoenzyme is involved in regulation of chromosome condensation at interphase as well as mitosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Cycle
  • Chromosomes / ultrastructure
  • Cloning, Molecular
  • Drosophila melanogaster / enzymology
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / growth & development
  • Genes
  • Larva
  • Mitosis*
  • Molecular Sequence Data
  • Mutation
  • Phosphoprotein Phosphatases / genetics*
  • Phosphoprotein Phosphatases / metabolism
  • Protein Phosphatase 1

Substances

  • Phosphoprotein Phosphatases
  • Protein Phosphatase 1